ECOS™ St Competent Cells [HB101 Derived]

Strain: HB101 Derived
Description
ECOS™ St Competent Cells is a strain derived from HB101 and suitable for cloning unstable inserts such as lentiviral DNA with LTR (Long terminal repeat).
ECOS™ Competent Cells are chemically competent cells, which can be used with quick transformation protocol and no need recovery step. There are two transformation efficiencies of this strain in the ECOS™ product line. The item FYE307 is suitable for general use. If you want to transform longer plasmids, plasmids with a kanamycin selection system, or get maximum quantity colonies, you have an alternative option, HYE007-S08, the efficiency is 1*109 cfu/μg. This product can perfectly replace Stbl3™ of Thermo Fisher or Endura™ of Lucigen.
Genotype
F– mcrB mrr hsdS20(rB– ,mB– ) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR ) xyl-5 λ– leu mtl-1
Features and Advantages
  • Suitable for lentivirus or retrovirus cloning
  • The highest efficiency in the same strains of chemically competent cells in the world
  • 6-minute operation protocol can achieve the highest efficiency
Applications
  • Lentivirus and retrovirus cloning
Efficiency

>1 x 108 cfu/ μg Recommended for:

  • Subcloning
  • General cloning

>1 x 109 cfu/ μg Recommended for:

  • Super high-efficiency cloning
  • Cloning lowest abundance transcripts
  • Gene library
  • Kanamycin-selection vectors
Specification

Store at # HYE007-S08-10VL (10 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# HYE007-S08-80VL (80 preps)

Efficiency >1 x 109 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE307-10VL (10 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE307-80VL (80 preps) 

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)
Manual ECOS Card