T&A Expression Kit

Product overview

Description

The T&A™ Expression Kit (C-terminal) (FYC201-10P) contains the T&A™ Expression Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3’-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™).

Use with ECOS™ Sonic™ competent cells for optimal performance!

Features
  • Clone and  express proteins in one step (use with ECOS™ Sonic™)
  • 5 minute ligation
  • Bacteriophage T7 promoter for high-level expression; primers sites incuded for sequencing convenience
  • C-terminal 6x His tag for detection or purification
  • High transformation efficiency
  • Two types of ligation buffers provided for your convenience
  • Ampicillin markers for antibiotic selection
Applications

  • Gene construction and protein expression.

Specifications

Store at

Specifications

#FYC201-10P (10 preps)

T&ATM Expression Kit  (C-terminal)

T&A Expression Vector  (C-terminal) 20 μl (25 ng/μl)
Control Insert DNA (C-terminal) 10 μl (10 ng/μl)
yT4 DNA Ligase (2U) 10 μl
10X Ligation Buffer A 50 μl
10X Ligation Buffer B 50 μl
Primer T7 promoter 50 μl (10μM)
Primer T7 terminator 50 μl (10μM)

 

#FYC211-10P (10 preps)

T&ATM Expression Vector (C-terminal)

T&A Expression Vector  (C-terminal) 20 μl (25 ng/μl)
Control Insert DNA (C-terminal) 10 μl (10 ng/μl)
Primer T7 promoter 50 μl (10μM)
Primer T7 terminator 50 μl (10μM)

Technical

DNA Sequence of Multiple Cloning Sites (MCS)

 

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Support/ Documents

Manual