Total RNA Kit (Tissue) 2.0

Description

The Total RNA Kit (Tissue) 2.0 is designed specifically for purifying total RNA from fresh animal tissue or paraffin-embedded tissue. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.

Features
• Format: spin column (centrifuge)
• Yield: Up to 30 μg

Sample
30 mg of fresh animal tissue, 25 mg of paraffin-embedded tissue

Applications
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis

TR Buffer	45 ml
W1 Buffer	45 ml
W2 Buffer	15 ml
Elution Buffer	10 ml
DNase Incubation Buffer	10 ml
RZ Column	100 pcs
Collection Tube	100 pcs

TR Buffer	125 ml
W1 Buffer	125 ml
W2 Buffer	25 ml x2
Elution Buffer	30 ml
DNase Incubation Buffer	30 ml
RZ Column	300 pcs
Collection Tube	300 pcs