YLEX & SCOS & Protein Marker publications

YLEX Yeast Expression Kit

1. Expression of AHPND toxin genes (pirAB), quorum sensing master regulator gene (luxR) and transmembrane transcriptional regulator gene (toxR) in Vibrio parahaemolyticus and Vibrio harveyi during infection of Penaeus vannamei (Bonne, 1931). Sarmila Muthukrishnan, Mohamed Shariff, M.Y. Ina-Salwany, Fatimah Md Yusoff, I. Natrah – Aquaculture, 2022

2. Prototyping Yarrowia lipolytica for industrial production of hyperthermophilic enzymes- a case of β-glucosidase (CelB) from Pyrococcus furiosus. Kurshedaktar M. Shaikh, Vijay Pawale, Vishwanath S. Khadye, Suraj Sharma, Annamma A. Odaneth – Biochemical Engineering Journal, 2021

3. Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement. Madzak, Catherine – Journal of Fungi, 2021

4. Yarrowia lipolytica engineering as a source of microbial cell factories. Catherine Madzak – Academic Press, 2021

5. Metabolic engineering of Yarrowia lipolytica for the production of isoprene. Shaikh KM, Odaneth AA. – Biotechnol Prog., 2021

6. Disruption of the Snf1Gene Enhances Cell Growth and Reduces the Metabolic Burden in Cellulase-Expressing and Lipid-Accumulating Yarrowia lipolytica. Wei H, Wang W, Knoshaug EP, Chen X, Van Wychen S, Bomble YJ, Himmel ME, Zhang M – Front Microbiol., 2021

7. Mapping enzyme catalysis with metabolic biosensing. Xu, L., Chang, KC., Payne, E.M. et al.Nature Communications, 2021

8. Disrupting a phospholipase A2gene increasing lipid accumulation in the oleaginous yeast Yarrowia lipolytica. Li JX, Xu J, Ruan JC, Meng HM, Su H, Han XF, Lu M, Li FL, Wang SA. – Appl Microbiol., 2021

9. Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study. Zhao, Y., Liu, S., Lu, Z. et al.Biotechnol Biofuels, 2021

10. A Golden-Gate Based Cloning Toolkit to Build Violacein Pathway Libraries in Yarrowia lipolytica. Yingjia Tong, Jingwen Zhou, Liang Zhang, and Peng Xu – ACS Publications, 2021

11. Microbial synthesis of wax esters. Ya-Hue Valerie Soong, Le Zhao, Na Liu, Peng Yu, Carmen Lopez, Andrew Olson, Hsi-Wu Wong, Zengyi Shao, Dongming Xie – Metabolic Engineering, 2021

12. Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast. Alahuhta, M., Xu, Q., Knoshaug, E.P. et al. Biotechnol Biofuels, 2021

13. Disrupting a phospholipase A2 gene increasing lipid accumulation in the oleaginous yeast Yarrowia lipolytica, Jiaxin Li, Jing Xu, Jianchang Ruan, Huimin Meng, Hang Su  Xingfeng Han, Ming Lu, Fuli Li, Shi’an Wang-Journal of Applied Microbiology,2020

14. Established and Upcoming Yeast Expression Systems, Burcu Gündüz Ergün, Damla Hüccetoğulları, Sibel Öztürk, Eda Çelik, Pınar Çalık-Methods in Molecular Biology ,2019

15. Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone, Hui Wei1-Frontiers in Microbiology,2019

16. Engineered microbes and methods for microbial oil production, Gregory Stephanopoulos, Mitchell Tai, Sagar Chakraborty-Massachusetts Institute of Technology, Cambridge, MA (United States),2018

17. Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags, Ewelina Celińska, Monika Borkowska, Wojciech Białas, Paulina Korpys, Jean-Marc Nicaud- Applied Microbiology and Biotechnology,2018

18. Engineering the architecture of erythritol-inducible promoters for regulated and enhanced gene expression in Yarrowia lipolytica. Young-Kyoung Park, Paulina Korpys , Monika Kubiak, Ewelina Celinska, Paul Soudier, Pauline Trébulle, Macarena Larroude, Tristan Rossignol, Jean-Marc Nicaud,2018

19. Expression of an endoglucanase–cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi, Qi Xu, Markus Alahuhta, Hui Wei, Eric P. Knoshaug, Wei Wang, John O. Baker, Todd Vander Wall, Michael E. Himmel, Min Zhang- Biotechnology for Biofuels,2018

20. Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations, Catherine Madzak- Molecular Biotechnology,2018

 21. YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in Yarrowia lipolytica, Lynn Wonga, Jake Engel, Erqing Jin, Benjamin Holdridge, Peng Xu-Metabolic Engineering Communications,2017

22. Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox, metabolism K Qiao- Nature Biotechnology, 2017

23. Enhanced production of insect raw-starch-digesting alpha-amylase accompanied by high erythritol synthesis in recombinant Yarrowia lipolytica fed-batch cultures at high-cell-densities, E Celińska – Process Biochemistry, 2017

24. Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica, P Xu- Biotechnology and Bioengineering, 2017

25. A molecular genetic toolbox for Yarrowia lipolytica, EL Bredeweg- Biotechnology for Biofuels, 2017

26. Evaluation of heterologous α-amylase production in two expression platforms dedicated for Yarrowia lipolytica: commercial Po1g–pYLSC (php4d) and custom-made, A18–pYLTEF (pTEF). Celińska , Borkowska , Białas –Yeast,2016

27. Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals, P Xu – PNAS, 2016

28. Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production, ZJ Li- Journal of Industrial Microbiology & Biotechnology, 2016

29. Functional overexpression and characterization of lipogenesis-related genes in the oleaginous yeast Yarrowia lipolytica , AM Silverman- Applied Microbiology and Biotechnology, 2016

30. ntegrated bioprocess for conversion of gaseous substrates to liquids, P Hu – Proceedings of the National Academy of Sciences of The United States Of, America (PNAS), 2016

31. The expression of the Cuphea palustris thioesterase CpFatB2 in Yarrowia lipolytica triggers oleic acid accumulation, A Stefan – Biotechnology progress, 2016

32. Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica, W Wang – Biotechnology for Biofuels, 2016

33. Characterization of phosphatidic acid phosphatase activity in the oleaginous yeast Yarrowia lipolytica and its role in lipid biosynthesis, D Hardman – Yeast, 2016

34. Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica, K Qiao- Metabolic Engineering ,2015

35. Yarrowia lipolytica: recent achievements in heterologous protein expression and, pathway engineeringC Madzak – Applied microbiology and biotechnology, 2015

36. Cloning, expression, and purification of insect (Sitophilus oryzae) alpha-amylase, able to digest granular starch, in Yarrowia lipolytica host, E Celińska – Applied microbiology and biotechnology, 2015

37. High-throughput transformation method for Yarrowia lipolytica mutant library, screening, C Leplat – FEMS yeast research, 2015

38. Biotechnological applications of Yarrowia lipolytica: past, present and future, HH Liu- Biotechnology Advances, 2015

39. Genetic engineering of non conventional yeast for the production of valuable compounds, A Sibirny – Microb Biotechnol Prog Trends, 2014

40. Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica, W Wang – PloS one, 2014

41. Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing, H Wei – Biotechnology for Biofuels, 2014

42. Biodiesel production under subcritical solvent condition using subcritical water treated whole Jatropha curcas seed kernels and possible use of hydrolysates to grow Yarrowia lipolytica, AW Go- Fuel, 2014

43. Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production, M Tai – Metabolic engineering, 2013

44. Bioethanol production from Yarrowia lipolytica Po1g biomass, YA Tsigie- Bioresource Technology, 2013

45. A study on the mechanism of subcritical water treatment to maximize extractable cellular lipids, PLT. Nguyen – Biomass and Bioenergy, 2013

46. Accumulation of lipid production in Chlorella minutissima by triacylglycerol biosynthesis-related genes cloned from Saccharomyces cerevisiae and Yarrowia lipolytica, HJ Hsieh – The Journal of Microbiology, 2012

47. Yarrowia lipolytica JM Nicaud – Yeast, 2012

48. Oil Production from Yarrowia lipolytica Po1g Using , Rice Bran Hydrolysate. YA Tsigie- Journal of Biomedicine and Biotechnology, 2012

49. Maximizing biodiesel production from Yarrowia lipolytica Po1g biomass using, subcritical water pretreatment. YA Tsigie – Bioresource technology, 2012

50. High efficiency transformation by electroporation of Yarrowia lipolytica, JH Wang – The Journal of Microbiology, 2011

51. Cloning and expression of a tyrosinase from Aspergillus oryzae in Yarrowia lipolytica: application in L-DOPA biotransformation, A Rao – Applied microbiology and biotechnology, 2011

52. Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate,YA Tsigie – Bioresource Technology, 2011

53. Co-expression of heterologous desaturase genes in Yarrowia lipolytica. LT Chuang – New biotechnology, 2010

54. Heterologous expression of Thermobifida fusca thermostable alpha-amylase in Yarrowia lipolytica and its application in boiling stable resistant sago starch preparation. CH Yang – Journal of Industrial Microbiology & Biotechnology, 2010


1.Direct fermentation of cellulose to ethanol by Saccharomyces cerevisiae displaying a bifunctional cellobiohydrolase gene from Orpinomyces sp. Y102. Jeng-Chen Liu, Wan-Jhu Chang, Teng-Chieh Hsu, Hui-Jye Chen, Yo-Chia Chen-Renewable Energy, 2020

Proteomics-Sharp Protein Marker III

1.Berberine Activates Aryl Hydrocarbon Receptor but Suppresses CYP1A1 Induction through miR-21-3p Stimulation in MCF-7 Breast Cancer Cells, Sheng-Nan Lo, Chun-Wei Wang, Yueh-Shieh Chen, Chiung-Chiao Huang, Tian-Shung Wu, Lih-Ann Li, I-Jung Lee, Yune-Fang Ueng-molecules,2017