EZtime Fast Real-Time PCR Premix

Product overview

Description

EZtime Fast Real-Time PCR Premix for SYBR® Green (#FYT108-100P/400P) , or the same premix with TaqMan® Probes (#FYT110-100P/400P), according to your preference, are ready-to-use, 2× concentrated premix reagents, containing all components except primers and template. They are formulated with a novel hot start Taq DNA polymerase, which is capable of catalyzing DNA amplification in a fast PCR mode. This special blend greatly shortens the running time of real-time quantitative PCR by around 1 hour when compared to traditional qPCR. In addition, it precisely meets current researchers’ needs for performing gene detection (qPCR) and quantification of gene expression (2-step qRT-PCR) in a high speed and/or high-throughput manner in addition to those basic requirements of high sensitivity, wide dynamic range, and good reproducibility. 

Features
• Fast: shortens the running time of real-time quantitative PCR by around 1 hour
• Specificity: Hotstart Taq and the optimized buffer eliminates non-specific amplification and formation of primer dimers
• Sensitivity: detects low copy number targets
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
Applications
• Quantitative real-time PCR
• Quantitative 2-step RT-PCR
• Quick and accurate detection and quantification of a target gene through real-time PCR

Specifications

Store at

Specifications
# FYT108-100P (100 preps)
EZtime Fast Real-Time PCR Premix
(2×, SYBR Green, ROX)		 1 ml

# FYT108-400P (400 preps)

EZtime Fast Real-Time PCR Premix
(2×, SYBR Green, ROX)		 1 ml x 4

# FYT110-100P (100 preps)

EZtime Fast Real-Time PCR Premix
(2×, TaqMan, ROX)		 1 ml

# FYT110-400P (400 preps)

EZtime Fast Real-Time PCR Premix
(2×, TaqMan, ROX)		 1 ml x 4

Technical

Quality Control
• Error<0.01, 2.1>Efficiency>1.90, ∆CP<1
• The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate quality tests. Functionally tested in qPCR for specificity, sensitivity, and reproducibility using serial dilutions of control genomic DNA template.

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Support/ Documents

Manual