The EZtime Real-Time PCR Premix (# FYT103-100P or # FYT104-100P) is a ready-to-use, 2× concentrated PCR premix, including Hotstart Taq, SYBR Green I, optimized reaction buffer and nucleotides (# FYT104-100P with ROX) for running quantitative real-time PCR, including qPCR and 2-step qRT-PCR, in the SYBR Green I detection format. SYBR Green Premix provides high sensitivity, wide dynamic range, and reproducibility for quantification.
The EZtime Real-Time PCR Premix (# FYT105-100P or # FYT106-100P) is a ready-to-use, 2× concentrated PCR premix that contains all reagents (except for primers, probe, and template) needed for running real-time PCR (# FYT106-100P with ROX). Real-time PCR is performed by addition of various probes, such as TaqMan, Molecular Beacon, etc. This product combines the high-performance Hotstart Taq with a buffer which provides good specificity, amplification efficiency for real-time PCR.
Features
Specificity : Hotstart Taq and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity : detects low copy number targets
Wide linear range : accurate quantification across 9 orders of magnitude
Reproducibility and convenience : ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
Applications
Quantitative real-time PCR
Quantitative 2-step RT-PCR
Quick and accurate detection and quantification of a target gene through real-time PCR
Specifications
Store at
Specifications
# FYT103-100P (100 preps)
EZtime Real-Time PCR Premix (2×, SYBR Green)
1.25ml
# FYT103-400P (400 preps)
EZtime Real-Time PCR Premix (2×, SYBR Green)
1.25ml×4
# FYT104 -100P (100 preps)
EZtime Real-Time PCR Premix (2×, SYBR Green, ROX)
1.25ml
# FYT104-400P (400 preps)
EZtime Real-Time PCR Premix (2×, SYBR Green, ROX)
1.25ml×4
# FYT105-100P (100 preps)
EZtime Real-Time PCR Premix (2×, for probes)
1.25ml
# FYT105-400P (400 preps)
EZtime Real-Time PCR Premix (2×, for probes)
1.25ml×4
# FYT106 -100P (100 preps)
EZtime Real-Time PCR Premix (2×, for probes, ROX)
1.25ml
# FYT106-400P (400 preps)
EZtime Real-Time PCR Premix (2×, for probes, ROX)
1.25ml×4
Premix contains:
■ Hotstart Taq DNA polymerase
■ Real-time PCR Buffer (FYT103, FYT104 including SYBR Green)
■ dNTP mix including dATP, dCTP, dGTP, dTTP
■ 5 mM MgCl2
Technical
Quality Control
Error<0.01, 2.1>Efficiency>1.90, ∆CP<1
The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate quality tests. Functionally tested in qPCR for specificity, sensitivity, and reproducibility using serial dilutions of control genomic DNA template.[su_divider]
