ECOS™ 21 Competent Cells [BL21(DE3)]

Product overview

Description
This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Genotype
F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
 
Features
  • lack of Lon and Omp proteases allows high-level protein expression and accumulation
  • Easy gene induction
Applications
  • IPTG induced gene expression in E. coli
  • Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
  • Subcloning

Technical / Specifications

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Genotype
F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Specifications

# FYE207-5VL (5 preps)

Efficiency >2 x 107 cfu/μg
100 μl/vial 5 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE207-40VL (40 preps)

Efficiency >2 x 107 cfu/μg
100 μl/vial 40 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE207-96WL (96 preps)

Efficiency >2 x 107 cfu/μg
50 μl/well 96wells

Support/ Documents

Manual ECOS Card