PCR publications


PCR Reagents

1. Sub-lethal concentration of sulfamethoxazole affects the growth performance of milkfish (Chanos chanos), the microbial composition of antibiotic-resistant bac teria and the prevalence of sulfonamide-resistance genes in mariculture. Lau SH, Hsu JT, Chen YJ, Li ZF, Chao WL, Yeh SL, Ying C. – Mar Pollut Bull., 2022

2. Fgf9-Nolz-1-Wnt2 Signaling Axis Regulates Morphogenesis of the Lung. Shih-Yun Chen, Fu-Chin Liu – bioRxiv, 2022

YEAtaq DNA Polymerase

1. Antimicrobial and Anticorrosion Activity of a Novel Composite Biocide against Mixed Bacterial Strains in Taiwanese Marine Environments. Chang SY, Huang SY, Chu YR, Jian SY, Lo KY, Lee YL. Materials (Basel), 2021

2. Method and device for polymerase chain reaction. Dar-Bin Shieh,Chih-Chia HUANG,Chen-Min Chang,Tsung-Ju LI,Po-Yang Chang,Ming-Chi HSIEH,2019

3. Quantitative analysis of genetically modified soya using multiple reaction monitoring mass spectrometry with endogenous peptides as internal standards, Shobha Devi, Yi-Cheng Lin and Yen-Peng Ho,2019

4. Molecular characterization, comparison of screening methods, and evaluation of crosspathogenicity of black rot (Xanthomonas campestris pv. campestris) strains from cabbage, choysum, leafy mustard, and pak choi from Taiwan, Rishi R Burlakoti, Jaw-rong Chen, Chiou-fen Hsu, Pragyan Burlakoti, Lawrence Kenyon,2018

5. Arsenic treatment increase Aurora-A overexpression through E2F1 activation in bladder cells. YT Kao, CH Wu, SY Wu, SH Lan, HS Liu- BMC Cancer, 2017

6. Mapping quantitative trait loci for fruit traits and powdery mildew resistance in melonYH Wang – Botanical Studies, 2016

7. Gene knockout of glutathione reductase 3 results in increased sensitivity to salt stress in rice. TM Wu – Plant molecular biology, 2015

8. Knock-out of glucose dehydrogenase gene in Gluconacetobacter xylinus for bacterial cellulose production enhancement. CH Kuo – Biotechnology and Bioprocess Engineering, 2015

9. Assessment of Genetic Variation in Different Kenaf (Hibiscus cannabinus) Genotypes using Morpho-Agronomic Traits and RAPD. G Faruq – International Journal of Agriculture & Biology, 2015

10. Hygromycin B-induced cell death is partly mediated by reactive oxygen species in rice (Oryza sativa L.). HM Oung – Plant molecular biology, 2015

11. Genome-wide InDel marker system for application in rice breeding and mapping studies. DH Wu – Euphytica, 2013

12. Decreased SPLUNC1 expression is associated with pseudomonas infection in surgically treated chronic rhinosinusitis patients who may require repeated sinus surgery. YA Tsou – The Laryngoscope, 2013

13. The-308G/A of tumor necrosis factor (TNF)-α and 825C/T of guanidine nucleotide binding protein 3 (GNB3) are Associated with the Onset of Acute Myocardial infarction and obesity in Taiwan. WT Chang – International journal of molecular sciences, 2012

14. Recombinant dioscorins of the yam storage protein expressed in Escherichia coli exhibit antioxidant and immunomodulatory activities. YJ Jheng – Protein expression and purification., 2012

15. A comparison of conventional and miniprimer PCR to elucidate bacteria diversity in Malaysia Ulu Slim hot spring using 16S rDNA clone library. KM Goh – Rom. Biotechnol. Lett, 2011

16. Characterization of tetracycline resistance lactobacilli isolated from swine intestines at western area of Taiwan. YC Chang – Anaerobe, 2011

17. Meiothermus sp. SK3-2: a potential source for the production of trehalose from maltose. KM Goh – A frican Journal of Biotechnology, 2011

18. Production of reactive oxygen species and induction of signaling pathways for the ACO gene expressions in tomato plants triggered by the volatile organic compound ether. KC Lin – Plant cell reports, 2011

19. The immuno-regulatory effects of Schisandra chinensis and its constituents on human monocytic leukemia cells. RD Lin – Molecules, 2011

20. Distinct clinical and biological features of de novo acute myeloid leukemia with additional sex comb-like 1 (ASXL1) mutations. WC Chou – Blood, 2010

21. Absence of nucleotide alteration in region of exon 34 of NOTCH1 and NOTCH2 receptor genes analysed in oral cancer samples: a preliminary observation. NN Ismail – Archives of Orofacial Sciences, 2010

22.The 252A/G and 804C/A polymorphisms of Lymphotoxin-alpha is associated to onset of acute myocardial infarction in Taiwan. YC Wang – Laboratory Medicine, 2010

23. Use of random amplified polymorphic DNA analysis and single-enzyme amplified fragment length polymorphism in molecular typing of Ornithobacterium rhinotracheale strains. CH Chou – Avian diseases, 2009

24. Extensive DNA sequence variations in two lignin genes, Cinnamate 4-hydroxylase and Cinnamyl Alcohol Dehydrogenase from Acacia mangium and Acacia mangium and Acacia auriculiformis.. MSN Fariza – Journal of Biological Sciences, 2008


O’in 1 DNA Polymerase Premix

1.Evaluation of onion (Allium cepa) germplasm entries for resistance to onion thrips, Thrips tabaci (Lindeman) in Tanzania, Gabriel Michael Njau, Agnes M. S. Nyomora, Fekadu Fufa Dinssa, Jian-Cheng Chang, P. Malini, S. Subramanian and R. Srinivasan-International Journal of Tropical Insect Science,2017

2.Integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan. Yi‑Chih Chang, Ni Tien, Jai‑Sing Yang , Chi‑Cheng Lu , Fuu‑Jen Tsai, Tsurng‑Juhn Huang and I‑Kuan Wang-Gut Pathogens ,2017

3.Characterization of tetracycline resistance lactobacilli isolated from swine intestines at western area of Taiwan. YC Chang – Anaerobe, 2011

4.Diazoxide ameliorates microcirculatory disturbances through PKC-dependent pathway in I/R-injured rat cremaster muscles. W Wei – Journal of biomedical science, 2005

5.Resveratrol protects myocardial ischemia–reperfusion injury through both NO-dependent and NO-independent mechanisms. LM Hung – Free Radical Biology and Medicine, 2004

6.Ischemic preconditioning ameliorates microcirculatory disturbance through downregulation of TNF-alpha production in a rat cremaster muscle model. LM Hung – Journal of biomedical science, 2004


RealStart DNA Polymerase Premix

  1. Genetic Analysis in a Taiwanese Cohort of 750 Index Patients with Clinically Diagnosed Familial Hypercholesterolemia. Huang CC, Niu DM, Charng MJ. – J Atheroscler Thromb, 2022

2. Deep-­sea anglerfishes (Lophiiformes: Ceratioidei) from the western North Atlantic: Testing the efficacy of DNA barcodes. Rafael Bañón, David Barros-García,JC s Arronte,AS Comesaña, Lucía Sánchez-Ruiloba,AD Carlos-Journal zoological systematics evolutionary research,2019

3. Desarrollo de métodos para la extracción de ADNmt de carpóforos de basidiomicetes. Sandra Ramos Pandiella,2018

4. First haplosporidan parasite reported infecting a member of the Superfamily Pinnoidea (Pinna nobilis) during a mortality event in Alicante. S Darriba – Journal of Invertebrate Pathology, 2017

5. A novel paramyxean parasite, Marteilia octospora n. sp.(Cercozoa) infecting the Grooved Razor Shell clam Solen marginatus from Galicia (NW Spain). M Ruiz – Journal of invertebrate pathology, 2016

6. Development and mapping of CL-repeat display markers on the maize B chromosome. YL Chien – Cytogenetic and genome research, 2015

7. Recognition of algae by microcontact-imprinted polymers modulates hydrogenase expression. MH Lee – RSC Advances, 2014


Direct PCR

1.A comparison of conventional and miniprimer PCR to elucidate bacteria diversity in Malaysia Ulu Slim hot  spring using 16S rDNA clone library.KM Goh, YS Chua, R Rahman, R Chan, RM Illias – Rom. Biotechnol. Lett, 2011

2.A Rapid and Accurate Method to Evaluate Helicobacter pylori Infection, Clarithromycin Resistance, and CYP2C19 Genotypes Simultaneously From Gastric. CH Kuo, CJ Liu, CC Yang, FC Kuo, HM Hu, HY Shih- Medicine-Romanian Biotechnological Letters , 2016

EZtime Real-Time PCR Premix

  1. Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a rabbit spine fusion model. Fu T, Chen W, Wang Y, et al. – Research Square, 2021
  2. INVESTIGATION OF EFFECTS OF PORCINE CIRCOVIRUS TYPE 2 (PCV2) VACCINATION ON CELLULAR IMMUNITY IN PIGS IN A TAIWAN PIG FARM. Yen-Chen Chang, Hui-Wen Chang, Hue-Ying Chiou, Chian-Ren Jeng, Victor Fei Pang, Yen-Chen Chang – Taiwan Veterinary Journal, 2021

3. Overexpression of the stress-associated protein (SAP) SAP^ sub MR219^ in Oryza sativa (var. MR219 increases salt tolerance in transformed Arabidopsis thaliana plants. P Hedayati – Plant OMICS, 2015

4. Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation. Cheng-Yi Yang , Ting-Hsuan Yu , Wan-Ling Wen , Pin Ling and Kuei-Sen Hsu-Journal of neuroscience,2019

5. Hydrogen peroxide inducible clone-5 sustains NADPH oxidase-dependent reactive oxygen species-c-jun N-terminal kinase signaling in hepatocellular carcinoma. Jia-Ru Wu, Ren-In You , Chi-Tan Hu, Chuan-Chu Cheng , Rudy Rudy and Wen-Sheng Wu-Oncogenesis,2019

6. Downregulation of nuclear and cytoplasmic Chibby is associated with advanced cervical cancer. MING-CHANG YANG, SHANG-TAO CHIEN, TZU-FENG YANG, SHIH-YI LIN , TAI-MIN LEE and YI-REN HONG-Oncology Letters,2017

7. Naloxone inhibits nod-like receptor protein 3 inflammasome. HY Lin, YY Chang, MC Kao, CJ Huang – Journal of Surgical Research, 2017

EZtime Fast Real-Time PCR Premix

1.MiR‑381 regulates cell motility, growth and colony formation through PIK3CA in endometriosis‑associated clear cell and endometrioid ovarian cancer, Chia‑Yi Hsu,  Tsung‑Hua Hsieh, Tze‑Kiong Er, Hung‑Sheng Chen, Ching‑Chou Tsai, Eing‑Mei Tsai-Oncology reports,2018

2.Association between HTR1B alleles and suicidal ideation in individuals with major depressive disorder. WT Kao – Neuroscience Letters, 2017

3.Diminished COX-2/PGE 2-Mediated Antiviral Response Due to Impaired NOX/MAPK Signaling in G6PD-Knockdown Lung Epithelial Cells. HR Lin – PloS one, 2016

4.Impacts of low temperature preservation on mitochondrial DNA copy number in oocytes of the hard coral Echinopora sp. S Tsai – Mitochondrial DNA Part A, 2016

5.Differential roles of Bcl2L12 and its short variant in breast cancer lymph node metastasis. ST Chien – Oncology reports, 2015

6.Glucose 6-phosphate dehydrogenase knockdown enhances IL-8 expression in HepG2 cells via oxidative stress and NF-κB signaling pathway. HC Yang – Journal of Inflammation-London, 2015

7.Recognition of Rhodobacter sphaeroides by microcontact-imprinted poly (ethylene-co-vinyl alcohol). MH Lee – Colloids And Surfaces B-Biointerfaces, 2015

8.Recognition of algae by microcontact-imprinted polymers modulates hydrogenase expression. MH Lee – RSC Advances, 2014

9.Rubisco expression in the dinoflagellate Symbiodinium sp. is influenced by both photoperiod and endosymbiotic lifestyle. AB Mayfield – Marine biotechnology, 2014

10.Compartment‐specific transcriptomics in a reef‐building coral exposed to elevated temperatures. AB Mayfield – Molecular ecology, 2014

11.The osteogenesis of bone marrow stem cells on mPEG-PCL-mPEG/hydroxyapatite composite scaffold via solid freeform fabrication. HT Liao – Biomed Research International, 2014

12.Rubisco expression in the dinoflagellate Symbiodinium sp. is influenced by both photoperiod and endosymbiotic lifestyle. AB Mayfield – Marine biotechnology, 2014

13.The effects of temperature on gene expression in the Indo-Pacific reef-building coral Seriatopora hystrix: insight from aquarium studies in Southern Taiwan. AB Mayfield – International Journal of Marine Science, 2014

14.Bone tissue engineering with adipose-derived stem cells in bioactive composites of laser-sintered porous polycaprolactone scaffolds and platelet-rich plasma. HT Liao – Materials, 2013

15.The physiological and molecular responses of larvae from the reef-building coral Pocillopora damicornis exposed to near-future increases in temperature and pCO2. HM Putnam – Marine Biology, 2013

16.Detection of EBV infection and gene expression in oral cancer from patients in Taiwan by microarray analysis. CY Yen – Biomed Research International, 2009