EtB”Out” Nucleic Acid Staining Solution aims to replace traditional EtBr (ethidium bromide) in performing nucleic acid detection in agarose gels. EtBr has long been known as a strong mutagen, however EtB”Out” causes only neglectable mutations in the Ames test.
The sensitivity of EtB”Out” is identical to that of EtBr. Under UV light, EtB”Out” emits green fluorescence when bound to DNA or RNA. EtB”Out” can be excited at 290 nm and 490 nm. The fluorescence emission peak of EtB”Out” when it bound to DNA is at 537 nm.