Description
The Total RNA Kit (Plant) 2.0 is designed specifically for purifying total RNA from plant tissue or dry plant tissue. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.
Features
• Format: spin column (centrifuge)
• Yield: Up to 30 μg
Sample
100 mg of plant Tissue, 25 mg of dry plant Tissue
Applications
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis