YB Rapid Ligation Kit
Description
Yeastern’s yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5′ – phosphate and the 3′ – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. YB Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.
Features
- Either sticky- or blunt-end, ligation can be achieved within 5 minutes
- The reaction mixture can be used directly for bacterial transformation
Applications
- Join double-strand DNA with cohesive or blunt termini
- Join oligonucleotide linkers to DNA sequence with the blunt end
- Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids
Combo Products
Quality Control
Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities; Undetectable ribonuclease activity; Undetectable degradation of labeled oligonucleotide; Passed the test of the capability to join cohesive- and blunt-ended DNA fragments.
Specification
Store at
# FYC003-100R (100 rxns)
| yT4 DNA Ligase | 100 μl (3 U/μl) |
| 10× Ligation Buffer A | 200 μl |
| 10× Ligation Buffer B | 200 μl |
Storage Buffer
| Tris-HCl (pH7.5) | 20 mM |
| KCl | 50 mM |
| DTT | 1 mM |
| EDTA | 0.1 mM |
| Glycerol stabilizers | 50% |
10× Ligation Buffer A
| Tris-HCl | 0.4M |
| MgCl2 | 0.1 mM |
| DTT | 0.1 mM |
| EDTA | 5 mM |
| ATP (pH5.0 at 25°C) | 5 mM |
| The performance of this buffer depends on the integrity of ATP. Store the buffer in small aliquots at -20°C to minimize degradation of ATP and DTT | |
10× Ligation Buffer B
The buffer contains an enhancer which dramatically increases ligation efficiency for blunt end DNA
