The T&A™ Expression Kit (C-terminal) (FYC201-10P) contains the T&A™ Expression Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3’-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™).
Use with ECOS™ Sonic™ competent cells for optimal performance!