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\u00a0<\/div>\n<\/div>\n
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Description<\/div>\n
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The\u00a0YLOS<\/em>\u00a0(Yarrowia lipolytica<\/em>\u00a0one-step) Transformation Kit provides a simple and fast one-step\/one-tube method to transform\u00a0Yarrowia<\/em>\u00a0cells cultured in either solid agar or liquid broth. They were designed for various strains of\u00a0Y. lipolytica<\/em>\u00a0and vectors. Transformation efficiencies with\u00a0Y. lipolytica<\/em>\u00a0will vary based on the yeast strain used, a linear or circular plasmid DNA, the efficiency of plasmid integration into the host chromosome, and the transformation procedure chosen. Generally, the entire procedure may be completed in 60 mins, and routinely provides a transformation efficiency of 103<\/sup>\u00a0~ 105<\/sup>\u00a0transformants\/\u03bcg of plasmid DNA.<\/div>\n<\/div>\n
Features<\/div>\n
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- Instant preparation of competent cells<\/li>\n
- An one-step, one-tube and 10 ~ 60 minutes protocol for transforming<\/li>\n
- the oleaginous yeast, Yarrowia lipolytica<\/li>\n
- Simple (Mix\u2192 Heat shock\u2192 Plating), suitable for Yarrowia yeast<\/li>\n
- Cells from colonies, broth or any growth phase<\/li>\n
- Repeatable efficiency, always reach efficiency of 103<\/sup>\u00a0~ 105<\/sup>transformants \/\u03bcg DNA<\/li>\n<\/ul>\n<\/div>\n<\/div>\nApplications<\/div>\n\n\n
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- Transformation of\u00a0Y. lipolytica<\/em><\/li>\n<\/ul>\n<\/div>\n<\/div>\nProtocol<\/div>\n\n
![\"\"](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/YLOS-PROTOCOL.jpg\")
<\/div>\n![\"\"](\"http:\/\/www.yeastern.com\/updfile\/upload\/image\/PIC018.png\")
<\/div>\n<\/div>\nQuality Control<\/div>\n\nThe transformation efficiency with the plasmid pYLEX1 is checked in a lot-to-lot basis to ensure that it is above minimum 103<\/sup>transformants\/\u03bcg of plasmid DNA.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\u00a0<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\nSpecification<\/div>\n\nStore at<\/span><\/p>\n
![](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/4C-300x222.png\")
<\/p>\n# FYY301-120P<\/b>\u00a0(120 preps)<\/span><\/p>\n
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- Transformation of\u00a0Y. lipolytica<\/em><\/li>\n<\/ul>\n<\/div>\n<\/div>\n
![\"\"](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\")
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