{"id":5585,"date":"2020-07-22T16:18:17","date_gmt":"2020-07-22T08:18:17","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=5585"},"modified":"2020-07-29T15:02:11","modified_gmt":"2020-07-29T07:02:11","slug":"yb","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=5585","title":{"rendered":"YB Rapid Ligation Kit"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; specialty=&#8221;on&#8221; custom_padding_last_edited=&#8221;on|desktop&#8221; padding_top_bottom_link_2=&#8221;false&#8221; padding_left_right_link_2=&#8221;false&#8221; padding_top_2=&#8221;0px&#8221; padding_bottom_2=&#8221;0px&#8221; _builder_version=&#8221;4.0.7&#8243; custom_padding=&#8221;0px||0px||false|false&#8221; custom_padding_tablet=&#8221;&#8221; custom_padding_phone=&#8221;&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_menu menu_id=&#8221;52&#8243; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.0.7&#8243; menu_font=&#8221;|600|||||||&#8221; menu_text_color=&#8221;#000000&#8243; menu_font_size=&#8221;15px&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;|||0px||&#8221;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; specialty_columns=&#8221;3&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_row_inner _builder_version=&#8221;4.0.6&#8243;][et_pb_column_inner saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.6&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243; hover_enabled=&#8221;0&#8243;]<\/p>\n<h1 class=\"entry-title main_title\">YB Rapid Ligation Kit<\/h1>\n<div class=\"entry-content\">\n<div id=\"pl-1386\" class=\"panel-layout\">\n<div id=\"pg-1386-0\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-1386-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1386-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div class=\"all_font\"><span>Description<\/span><\/div>\n<\/div>\n<div>\n<div>\n<div>Yeastern\u2019s yT<sub>4<\/sub>\u00a0DNA ligase catalyzes the joining of two strands of DNA between the 5\u2032 \u2013 phosphate and the 3\u2032 \u2013 hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA\/RNA hybrids. YB Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.<\/div>\n<\/div>\n<div>\u00a0<\/div>\n<div>Features<\/div>\n<div>\n<div>\n<ul>\n<li>Either sticky- or blunt-end, ligation can be achieved within 5 minutes<\/li>\n<li>The reaction mixture can be used directly for bacterial transformation<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div>Applications<\/div>\n<div>\n<ul>\n<li>Join double-strand DNA with cohesive or blunt termini<\/li>\n<li>Join oligonucleotide linkers to DNA sequence with the blunt end<\/li>\n<li>Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-2839\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Ligation-kit-efficency.jpg\" alt=\"\" width=\"992\" height=\"828\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Ligation-kit-efficency.jpg 1449w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Ligation-kit-efficency-768x641.jpg 768w\" sizes=\"(max-width: 992px) 100vw, 992px\" \/><\/p>\n<p>Combo Products<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-2840\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Ligation-combo-products.jpg\" alt=\"\" width=\"1086\" height=\"374\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Ligation-combo-products.jpg 2213w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Ligation-combo-products-768x264.jpg 768w\" sizes=\"(max-width: 1086px) 100vw, 1086px\" \/><\/p>\n<\/div>\n<div><span>Quality Control<\/span><\/div>\n<div>\n<div>Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities; Undetectable ribonuclease activity; Undetectable degradation of labeled oligonucleotide; Passed the test of the capability to join cohesive- and blunt-ended DNA fragments.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1386-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1386-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">Specification<\/div>\n<div class=\"all_font\">\n<div>\n<p><span>Store at<\/span><\/p>\n<div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2357 size-medium\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-300x222.png\" alt=\"\" width=\"107\" height=\"79\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-300x222.png 300w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-230x170.png 230w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C.png 327w\" sizes=\"(max-width: 107px) 100vw, 107px\" \/><\/p>\n<p><span><b># FYC003-100R (100 rxns)<\/b><\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>yT<sub>4<\/sub>\u00a0DNA Ligase<\/td>\n<td align=\"right\">100 \u03bcl (3 U\/\u03bcl)<\/td>\n<\/tr>\n<tr>\n<td>10\u00d7 Ligation Buffer A<\/td>\n<td align=\"right\">200 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>10\u00d7 Ligation Buffer B<\/td>\n<td align=\"right\">200 \u03bcl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b><sub>Storage Buffer<\/sub><\/b><\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Tris-HCl (pH7.5)<\/td>\n<td align=\"right\">20 mM<\/td>\n<\/tr>\n<tr>\n<td>KCl<\/td>\n<td align=\"right\">50 mM<\/td>\n<\/tr>\n<tr>\n<td>DTT<\/td>\n<td align=\"right\">1 mM<\/td>\n<\/tr>\n<tr>\n<td>EDTA<\/td>\n<td align=\"right\">0.1 mM<\/td>\n<\/tr>\n<tr>\n<td>Glycerol stabilizers<\/td>\n<td align=\"right\">50%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b><sub>10\u00d7 Ligation Buffer A<\/sub><\/b><\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Tris-HCl<\/td>\n<td align=\"right\">0.4M<\/td>\n<\/tr>\n<tr>\n<td>MgCl<sub>2<\/sub><\/td>\n<td align=\"right\">0.1 mM<\/td>\n<\/tr>\n<tr>\n<td>DTT<\/td>\n<td align=\"right\">0.1 mM<\/td>\n<\/tr>\n<tr>\n<td>EDTA<\/td>\n<td align=\"right\">5 mM<\/td>\n<\/tr>\n<tr>\n<td>ATP (pH5.0 at 25\u00b0C)<\/td>\n<td align=\"right\">5 mM<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\">The performance of this buffer depends on the integrity of ATP. Store the buffer in small aliquots at -20\u00b0C to minimize degradation of ATP and DTT<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b><sub>10\u00d7 Ligation Buffer B<\/sub><\/b><\/span><\/p>\n<div>The buffer contains an enhancer which dramatically increases ligation efficiency for blunt end DNA<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div><\/div>\n<div><\/div>\n<div class=\"all_font\">\n<div>\n<div>\n<div>\n<table width=\"1679\">\n<tbody>\n<tr>\n<td>Manual<\/td>\n<\/tr>\n<tr>\n<td><a href=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/09\/FYO001-100G-Glass-Plating-Beads-Sterilized-Manual.pdf\">\u00a0\u00a0<\/a><a href=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/YB-Rapid-Ligation-kit-For-Cloning.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2535\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>YB Rapid Ligation Kit Description Yeastern\u2019s yT4\u00a0DNA li [&hellip;]<\/p>\n","protected":false},"author":27,"featured_media":2230,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-5585","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5585","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5585"}],"version-history":[{"count":10,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5585\/revisions"}],"predecessor-version":[{"id":5681,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5585\/revisions\/5681"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/media\/2230"}],"wp:attachment":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5585"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}