{"id":5470,"date":"2020-07-20T14:48:20","date_gmt":"2020-07-20T06:48:20","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=5470"},"modified":"2020-09-01T13:38:11","modified_gmt":"2020-09-01T05:38:11","slug":"reverse-transcriptase-pcr-qpcrs-2","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=5470","title":{"rendered":"Reverse Transcriptase,RT-PCR\/RT-qPCRs"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; specialty=&#8221;on&#8221; _builder_version=&#8221;4.0.7&#8243; custom_padding=&#8221;||26px|||&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_menu menu_id=&#8221;52&#8243; menu_style=&#8221;centered&#8221; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.0.7&#8243; menu_font=&#8221;|600|||||||&#8221; menu_text_color=&#8221;#000000&#8243; menu_font_size=&#8221;16px&#8221; custom_margin=&#8221;||||false|false&#8221; custom_margin_tablet=&#8221;&#8221; custom_margin_phone=&#8221;&#8221; custom_margin_last_edited=&#8221;on|desktop&#8221;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; specialty_columns=&#8221;3&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_row_inner _builder_version=&#8221;4.0.7&#8243; custom_padding=&#8221;0px|||||&#8221;][et_pb_column_inner saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243;]<\/p>\n<div class=\"et_pb_module et_pb_text et_pb_text_0  et_pb_text_align_left et_pb_bg_layout_light\">\n<div class=\"et_pb_text_inner\">\n<h1 style=\"text-align: center;\"><span style=\"text-decoration: underline;\"><strong><\/strong><\/span><\/h1>\n<h1 style=\"text-align: center;\"><span style=\"text-decoration: underline;\"><strong><\/strong><\/span><\/h1>\n<h1 style=\"text-align: center;\"><span style=\"text-decoration: underline;\"><strong><\/strong><\/span><\/h1>\n<h1 style=\"text-align: center;\"><span style=\"text-decoration: underline;\"><strong><\/strong><\/span><\/h1>\n<h1 style=\"text-align: center;\"><span style=\"text-decoration: underline;\"><strong>Reverse Transcriptase, RT-PCR\/RT-qPCRs<\/strong><\/span><\/h1>\n<p><span style=\"text-decoration: underline;\"><strong><\/strong><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><\/strong><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><\/strong><\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3007\" src=\"\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Reverse-transcriptase-selection.png\" alt=\"\" width=\"1885\" height=\"417\" srcset=\"\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Reverse-transcriptase-selection.png 1885w, \/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Reverse-transcriptase-selection-768x170.png 768w\" sizes=\"(max-width: 1885px) 100vw, 1885px\" style=\"font-size: 14px;\" \/><span style=\"font-size: 14px;\">\u00a0<\/span><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2996\" src=\"\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/comparison-table-of-reverse-transcriptase.png\" alt=\"\" width=\"1840\" height=\"580\" srcset=\"\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/comparison-table-of-reverse-transcriptase.png 1840w, \/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/comparison-table-of-reverse-transcriptase-768x242.png 768w\" sizes=\"(max-width: 1840px) 100vw, 1840px\" style=\"font-size: 14px;\" \/><\/p>\n<\/div>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.0.6&#8243; width=&#8221;100%&#8221; min_height=&#8221;297px&#8221; custom_margin=&#8221;0px|0px|-22px|0px|false|false&#8221; custom_padding=&#8221;22px|0px|0px|0px|false|false&#8221; border_radii=&#8221;on|NaNpx|NaNpx|NaNpx|NaNpx&#8221;][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.6&#8243; custom_padding=&#8221;|7px||7px|false|false&#8221; border_radii=&#8221;on|11px|11px|11px|11px&#8221; border_width_all=&#8221;3px&#8221; border_color_all=&#8221;#000000&#8243; border_style_all=&#8221;ridge&#8221;][et_pb_text _builder_version=&#8221;4.0.7&#8243; text_text_color=&#8221;#0c71c3&#8243; header_3_text_color=&#8221;#0c71c3&#8243;]<\/p>\n<h3 style=\"text-align: center;\"><span><strong><\/strong><\/span><\/h3>\n<h3 style=\"text-align: center;\"><span><strong><\/strong><\/span><\/h3>\n<h3 style=\"text-align: center;\"><span><strong>1-Step<\/strong><\/span><\/h3>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; width=&#8221;100%&#8221; min_height=&#8221;229px&#8221; custom_padding=&#8221;|0px||||&#8221; link_option_url=&#8221;http:\/\/si.secda.info\/yb_md\/?page_id=1411&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221;]<\/p>\n<h2><strong><\/strong><\/h2>\n<h3 style=\"text-align: center;\"><strong><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1411\">Deoxy+ OneStep RT-PCR Kit<\/a><\/strong><\/h3>\n<p><span><strong><sup><\/sup><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span><strong><sup><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/one-step.jpg\" width=\"330\" height=\"294\" alt=\"\" class=\"wp-image-5784 alignnone size-full\" \/><\/sup><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><strong>Less contamination &amp; Easy<\/strong><\/p>\n<p style=\"text-align: center;\"><strong> &amp; High sensitivity<\/strong><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong>One tube for reverse-transcription and PCR !<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span><strong><sup><\/sup><\/strong><\/span><\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; custom_padding=&#8221;||23px|||&#8221; link_option_url=&#8221;http:\/\/si.secda.info\/yb_md\/?page_id=1413&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221; inline_fonts=&#8221;Georgia&#8221;]<\/p>\n<h2><strong><\/strong><\/h2>\n<h3 style=\"text-align: center;\"><strong><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1413\">Deoxy+ Real-time SYBR Green RT-PCR Kit<\/a><\/strong><\/h3>\n<p><strong><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/sybr-green.jpg\" width=\"327\" height=\"287\" alt=\"\" class=\"wp-image-5785 alignnone size-full\" \/><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><span lang=\"EN-US\">Less contamination &amp;<\/span><span lang=\"EN-US\" style=\"font-family: 'Times New Roman',serif;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><span lang=\"EN-US\">Accurate detection<\/span><\/strong><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium; font-family: inherit; font-weight: normal;\"><strong><span lang=\"EN-US\">Excellent sensitivity and\u00a0easy\u00a0optimization\u00a0with\u00a0SYBR Green !<\/span><\/strong><\/span><\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; min_height=&#8221;188px&#8221; link_option_url=&#8221;http:\/\/si.secda.info\/yb_md\/?page_id=1415&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221;]<\/p>\n<h2><strong><\/strong><\/h2>\n<h3 style=\"text-align: center;\"><strong>Deoxy+ Fast One-Step\u00a0<\/strong><\/h3>\n<h3 style=\"text-align: center;\"><strong>qRT-PCR kit<\/strong><\/h3>\n<h3 style=\"text-align: center;\"><strong><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/deoxy-fast-one-step.jpg\" width=\"991\" height=\"855\" alt=\"\" class=\"wp-image-5790 alignnone size-full\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/deoxy-fast-one-step.jpg 991w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/deoxy-fast-one-step-980x846.jpg 980w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/deoxy-fast-one-step-480x414.jpg 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) 991px, 100vw\" \/><\/strong><\/h3>\n<p style=\"text-align: center;\"><strong><span>Less contamination &amp;<\/span><\/strong><strong><span>\u00a0Fast<\/span><\/strong><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong>RNA screening in one tube less than 1hr !<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong><\/strong><\/span><\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.0.7&#8243; custom_padding=&#8221;10px||8px|||&#8221;][\/et_pb_text][\/et_pb_column_inner][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.6&#8243; custom_padding=&#8221;|7px||7px|false|true&#8221; border_radii=&#8221;on|11px|11px|11px|11px&#8221; border_width_all=&#8221;3px&#8221; border_color_all=&#8221;#000000&#8243; border_style_all=&#8221;ridge&#8221;][et_pb_text _builder_version=&#8221;4.0.7&#8243; header_3_text_color=&#8221;#0c71c3&#8243; width=&#8221;96.8%&#8221;]<\/p>\n<h3 style=\"text-align: center;\"><strong><\/strong><\/h3>\n<h3 style=\"text-align: center;\"><strong><\/strong><\/h3>\n<h3 style=\"text-align: center;\"><strong>2-Step<\/strong><\/h3>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; width=&#8221;100%&#8221; min_height=&#8221;234px&#8221; custom_margin=&#8221;||36px|||&#8221; custom_padding=&#8221;||44px|||&#8221; link_option_url=&#8221;http:\/\/si.secda.info\/yb_md\/?page_id=1425&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221;]<\/p>\n<h2><strong><\/strong><\/h2>\n<h3 style=\"text-align: center;\"><strong><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1425\">Deoxy+ HiSpec Reverse Transcriptase\/ Deoxy+ RT Kit<\/a><\/strong><\/h3>\n<p><strong><\/strong><\/p>\n<p><strong><\/strong><\/p>\n<div>\n<h2><strong><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/HiSpec.png\" width=\"317\" height=\"282\" alt=\"\" class=\"wp-image-5466 alignnone size-full\" style=\"display: block; margin-left: auto; margin-right: auto;\" \/><\/strong><\/h2>\n<p style=\"text-align: center;\"><strong><\/strong><\/p>\n<p style=\"text-align: center;\"><strong>Thermolstable<\/strong><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong>Half-life of 100 minutes at 50\u00b0C for the highest cDNA yields !<\/strong><\/span><\/p>\n<\/div>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.0.6&#8243; custom_padding=&#8221;||817px|||&#8221;]<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Reverse Transcriptase, RT-PCR\/RT-qPCRs \u00a0 1-Step Deoxy+  [&hellip;]<\/p>\n","protected":false},"author":27,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<div id=\"pl-1194\" class=\"panel-layout\">\r\n<div id=\"pg-1194-0\" class=\"panel-grid panel-no-style\" data-style=\"{\"background_display\":\"tile\",\"cell_alignment\":\"flex-start\"}\" data-ratio=\"1\" data-ratio-direction=\"right\">\r\n<div id=\"pgc-1194-0-0\" class=\"panel-grid-cell\" data-weight=\"0.30468687384189\">\r\n<div id=\"panel-1194-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{\"background_image_attachment\":false,\"background_display\":\"tile\"}\">\r\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n\r\nPCR\r\n<ul id=\"menu-pcr\" class=\"menu\">\r\n \t<li id=\"menu-item-1481\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1481\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1455\">YEAtaq DNA Polymerase<\/a><\/li>\r\n \t<li id=\"menu-item-1480\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1480\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1457\">O\u2019in 1 DNA Polymerase Premix<\/a><\/li>\r\n \t<li><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=3276\">O\u2019in 1 DNA Polymerase Premix II<\/a><\/li>\r\n \t<li id=\"menu-item-1479\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1479\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1459\">Accu DNA Polymerase<\/a><\/li>\r\n \t<li id=\"menu-item-1478\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1478\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1461\">HiFi DNA Polymerase<\/a><\/li>\r\n \t<li id=\"menu-item-1475\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1475\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1471\">RealStart DNA Polymerase Premix<\/a><\/li>\r\n \t<li id=\"menu-item-1476\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1476\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1469\">Direct PCR Kits for Blood\/Plants<\/a><\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div id=\"pgc-1194-0-1\" class=\"panel-grid-cell\" data-weight=\"0.69531312615811\">\r\n<div id=\"panel-1194-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{\"background_display\":\"tile\"}\">\r\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n\r\n<img class=\"alignnone wp-image-2956\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Selection-Guide-of-YB-DNA-polymerase-goose.jpg\" alt=\"\" width=\"548\" height=\"586\" \/>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>","_et_gb_content_width":"","footnotes":""},"class_list":["post-5470","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5470","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5470"}],"version-history":[{"count":27,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5470\/revisions"}],"predecessor-version":[{"id":6117,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5470\/revisions\/6117"}],"wp:attachment":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5470"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}