{"id":5380,"date":"2020-07-15T09:13:42","date_gmt":"2020-07-15T01:13:42","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=5380"},"modified":"2020-08-07T14:04:48","modified_gmt":"2020-08-07T06:04:48","slug":"%e5%b7%a6%e6%ac%84-4","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=5380","title":{"rendered":"YEAtaq II DNA Polymerase"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; specialty=&#8221;on&#8221; _builder_version=&#8221;4.0.7&#8243; custom_padding=&#8221;||26px|||&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_menu menu_id=&#8221;52&#8243; menu_style=&#8221;centered&#8221; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.0.7&#8243; menu_font=&#8221;|600|||||||&#8221; menu_text_color=&#8221;#000000&#8243; menu_font_size=&#8221;16px&#8221; custom_margin=&#8221;||||false|false&#8221; custom_margin_tablet=&#8221;&#8221; custom_margin_phone=&#8221;&#8221; custom_margin_last_edited=&#8221;on|desktop&#8221;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; specialty_columns=&#8221;3&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_row_inner _builder_version=&#8221;4.0.6&#8243;][et_pb_column_inner saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.6&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243; hover_enabled=&#8221;0&#8243;]<\/p>\n<div id=\"pgc-1455-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1455-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div>\n<div class=\"all_font\"><strong><span>Description<\/span><\/strong><\/div>\n<\/div>\n<div>\n<div>\n<div>\n<p><em>YEA<\/em>taq II DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium\u00a0<em>Thermus aquaticus<\/em>. It is able to withstand repeated heating to 95\u00b0C without significant loss of activity. The enzyme catalyzes 5\u2019\u21923\u2019 synthesis of DNA, has no detectable proofreading 3\u2019\u21925\u2019 exonuclease activity and possesses low 5\u2019\u21923\u2019 exonuclease activity. It also exhibits deoxynucleotidyl transferase activity, which normally results in the addition of extra adenines at the 3\u2019-end of PCR products, required for the DNA ligation to TA vector.<\/p>\n<p>Yeastern Biotech offers\u00a0<em>YEA<\/em>taq II DNA Polymerase in two different packages: one with dNTPs mix and the other without. In addition, a lyophilized version of this enzyme will be on the market later soon this year.<\/p>\n<p>The enzyme is in a recombinant form expressed in\u00a0<em>E. coli<\/em>.<\/p>\n<\/div>\n<\/div>\n<div><strong>Features<\/strong><\/div>\n<div>\n<div>\n<ul>\n<li>Thermostable: half-life is more than 40 min at 95\u00b0C.<\/li>\n<li>10\u00d7 Reaction Buffer is supplied with two components: KCl and (NH<sub>4<\/sub>)<sub>2<\/sub>SO<sub>4<\/sub>, the latter allows for PCR at a wide range of magnesium concentrations and decreases non-specific priming.<\/li>\n<li>Incorporates modified nucleotides (e.g., dUTP, dITP, biotin-, digoxigenin-,fluorescently-labeled nucleotides).<\/li>\n<li>The error rate of YEAtaq DNA Polymerase in PCR is 2\u00d710<sup>-5<\/sup>errors per nucleotide per cycle.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div><strong>Applications<\/strong><\/div>\n<div>\n<div>\n<ul>\n<li>Cloning<\/li>\n<li>Screening<\/li>\n<li>Primer extension<\/li>\n<li>Terminal dA tailing<\/li>\n<li>Routine PCR amplification of DNA fragments up to 3 Kb<\/li>\n<li>DNA labeling<\/li>\n<li>DNA sequencing<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div><strong>Unit Definition<\/strong><\/div>\n<div>\n<div>One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into an acid-insoluble material in 30 minutes at 72\u00b0C.<\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<\/div>\n<div><\/div>\n<div><strong>Quality Control<\/strong><\/div>\n<div>\n<div>\n<ul>\n<li>Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb \/HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C.<\/li>\n<li>The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div><strong>Patterns\/Disclaimer<\/strong><\/div>\n<div>\n<div>\n<p>Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because the purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.<\/p>\n<p><strong>Specification<\/strong><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1455-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1455-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<p><span>Store at<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2357\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-300x222.png\" alt=\"\" width=\"124\" height=\"92\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-300x222.png 300w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-230x170.png 230w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C.png 327w\" sizes=\"(max-width: 124px) 100vw, 124px\" \/><\/p>\n<p><span><b># FYT601-500U<\/b>\u00a0(500 units)\u00a0<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td>YEAtaq II DNA Polymerase<br \/> (5 U\/\u03bcl)<\/td>\n<td align=\"right\">100 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>10\u00d7 Reaction Buffer<\/td>\n<td align=\"right\">1 ml<\/td>\n<\/tr>\n<tr>\n<td>dNTPs Mix \u00a0(10 mM)<\/td>\n<td align=\"right\">200 \u03bcl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYT611-500U<\/b>\u00a0(500units)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td>YEAtaq II DNA Polymerase<br \/> (5 U\/\u03bcl)<\/td>\n<td align=\"right\">100 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>10\u00d7 Reaction Buffer<\/td>\n<td align=\"right\">1 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b>Storage Buffer<\/b><\/span><\/p>\n<div>20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 0.5% Tween 20, 1 mM DTT, 50% Glycerol, 0.5% NP-40, Stabilizers<\/div>\n<p><span><b>10\u00d7 Reaction Buffer*\u00a0<\/b><\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td>\n<p>15 mM MgCl2, Tris-HCl (pH 8.3), (NH4)2SO4, KCl<\/p>\n<p>* The reaction buffer is supplied as a 10\u00d7 concentrate and should be diluted before use.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<table width=\"1679\">\n<tbody>\n<tr>\n<td>Manual<\/td>\n<\/tr>\n<tr>\n<td><a href=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/09\/FYO001-100G-Glass-Plating-Beads-Sterilized-Manual.pdf\">\u00a0\u00a0<\/a><a href=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/04\/FYT601-vT0302-compressed.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2535\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Description YEAtaq II DNA Polymerase is a thermostable  [&hellip;]<\/p>\n","protected":false},"author":27,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<div id=\"pl-1194\" class=\"panel-layout\">\r\n<div id=\"pg-1194-0\" class=\"panel-grid panel-no-style\" data-style=\"{\"background_display\":\"tile\",\"cell_alignment\":\"flex-start\"}\" data-ratio=\"1\" data-ratio-direction=\"right\">\r\n<div id=\"pgc-1194-0-0\" class=\"panel-grid-cell\" data-weight=\"0.30468687384189\">\r\n<div id=\"panel-1194-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{\"background_image_attachment\":false,\"background_display\":\"tile\"}\">\r\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n\r\nPCR\r\n<ul id=\"menu-pcr\" class=\"menu\">\r\n \t<li id=\"menu-item-1481\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1481\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1455\">YEAtaq DNA Polymerase<\/a><\/li>\r\n \t<li id=\"menu-item-1480\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1480\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1457\">O\u2019in 1 DNA Polymerase Premix<\/a><\/li>\r\n \t<li><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=3276\">O\u2019in 1 DNA Polymerase Premix II<\/a><\/li>\r\n \t<li id=\"menu-item-1479\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1479\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1459\">Accu DNA Polymerase<\/a><\/li>\r\n \t<li id=\"menu-item-1478\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1478\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1461\">HiFi DNA Polymerase<\/a><\/li>\r\n \t<li id=\"menu-item-1475\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1475\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1471\">RealStart DNA Polymerase Premix<\/a><\/li>\r\n \t<li id=\"menu-item-1476\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1476\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1469\">Direct PCR Kits for Blood\/Plants<\/a><\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div id=\"pgc-1194-0-1\" class=\"panel-grid-cell\" data-weight=\"0.69531312615811\">\r\n<div id=\"panel-1194-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{\"background_display\":\"tile\"}\">\r\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n\r\n<img class=\"alignnone wp-image-2956\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Selection-Guide-of-YB-DNA-polymerase-goose.jpg\" alt=\"\" width=\"548\" height=\"586\" \/>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>","_et_gb_content_width":"","footnotes":""},"class_list":["post-5380","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5380","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5380"}],"version-history":[{"count":6,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5380\/revisions"}],"predecessor-version":[{"id":6084,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5380\/revisions\/6084"}],"wp:attachment":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5380"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}