{"id":5069,"date":"2020-07-09T15:31:49","date_gmt":"2020-07-09T07:31:49","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=5069"},"modified":"2020-07-21T13:10:54","modified_gmt":"2020-07-21T05:10:54","slug":"home-23","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=5069","title":{"rendered":"Total RNA Kit (Blood\/ Bacteria)"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; specialty=&#8221;on&#8221; custom_padding_last_edited=&#8221;on|desktop&#8221; padding_top_bottom_link_2=&#8221;false&#8221; padding_left_right_link_2=&#8221;false&#8221; padding_top_2=&#8221;0px&#8221; padding_bottom_2=&#8221;0px&#8221; _builder_version=&#8221;4.0.7&#8243; custom_padding=&#8221;0px||0px||false|false&#8221; custom_padding_tablet=&#8221;&#8221; custom_padding_phone=&#8221;&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_menu menu_id=&#8221;52&#8243; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.0.7&#8243; menu_font=&#8221;|600|||||||&#8221; menu_text_color=&#8221;#000000&#8243; menu_font_size=&#8221;15px&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;|||0px||&#8221;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; specialty_columns=&#8221;3&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_row_inner _builder_version=&#8221;4.0.7&#8243;][et_pb_column_inner saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243; hover_enabled=&#8221;0&#8243;]<\/p>\n<h1 class=\"entry-title main_title\">Total RNA Kit (Blood\/ Bacteria)<\/h1>\n<div class=\"entry-content\">\n<div id=\"pl-1532\" class=\"panel-layout\">\n<div id=\"pg-1532-0\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-1532-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1532-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div>\n<div class=\"all_font\"><strong>Description<\/strong><\/div>\n<\/div>\n<div>\n<div>\n<p>The Total RNA kit (Blood\/ Bacteria) is designed specifically for purifying total RNA from fresh whole human blood and Bacteria. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.<\/p>\n<p><strong>Features<\/strong><br \/> \u2022 Format : spin column (centrifuge)<br \/> \u2022 Yield : Up to 30 \u03bcg<\/p>\n<p><strong>Sample<\/strong><br \/> 10<sup>9<\/sup><span>\u00a0<\/span>x Bacteria Cells, 300 \u03bcl of blood<\/p>\n<p><strong>Applications<\/strong><br \/> RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"all_font\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1532-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1532-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<p><strong>Specification<\/strong><\/p>\n<p>Store at<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2346\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/RT-300x222.png\" alt=\"\" width=\"125\" height=\"93\" srcset=\"https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/RT-300x222.png 300w, https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/RT-230x170.png 230w, https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/RT.png 327w\" sizes=\"(max-width: 125px) 100vw, 125px\" \/><\/p>\n<p><strong># FYG306-100<span>\u00a0<\/span><\/strong>(100 preps)<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"127\">BR1 Buffer<\/td>\n<td width=\"83\">110 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"127\">BR2 Buffer<\/td>\n<td width=\"83\">45 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"127\">BR3 Buffer<\/td>\n<td width=\"83\">25 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"127\">W1 Buffer<\/td>\n<td width=\"83\">45 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"127\">W2 Buffer<\/td>\n<td width=\"83\">15 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"127\">Elution Buffer<\/td>\n<td width=\"83\">10 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"127\">RZ Column<\/td>\n<td width=\"83\">100 pcs<\/td>\n<\/tr>\n<tr>\n<td width=\"127\">Collection Tube<\/td>\n<td width=\"83\">100 pcs<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong># FYG306-300<span>\u00a0<\/span><\/strong>(300 preps)<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"116\">BR1 Buffer<\/td>\n<td width=\"94\">100 ml x 3<\/td>\n<\/tr>\n<tr>\n<td width=\"116\">BR2 Buffer<\/td>\n<td width=\"94\">125 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"116\">BR3 Buffer<\/td>\n<td width=\"94\">65 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"116\">W1 Buffer<\/td>\n<td width=\"94\">125 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"116\">W2 Buffer<\/td>\n<td width=\"94\">25 ml x2<\/td>\n<\/tr>\n<tr>\n<td width=\"116\">Elution Buffer<\/td>\n<td width=\"94\">30 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"116\">RZ Column<\/td>\n<td width=\"94\">300 pcs<\/td>\n<\/tr>\n<tr>\n<td width=\"116\">Collection Tube<\/td>\n<td width=\"94\">300 pcs<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table width=\"1679\">\n<tbody>\n<tr>\n<td>Manual<\/td>\n<\/tr>\n<tr>\n<td><a href=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/09\/FYO001-100G-Glass-Plating-Beads-Sterilized-Manual.pdf\">\u00a0\u00a0<\/a><a href=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/FYG-306-Total-RNA-Kit-Blood-Bacteria.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2535\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.0.7&#8243; min_height=&#8221;157px&#8221;][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; fullwidth=&#8221;on&#8221; disabled_on=&#8221;on|on|on&#8221; _builder_version=&#8221;4.0.7&#8243; disabled=&#8221;on&#8221;][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Total RNA Kit (Blood\/ Bacteria) Description The Total R [&hellip;]<\/p>\n","protected":false},"author":26,"featured_media":2230,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-5069","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5069","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5069"}],"version-history":[{"count":5,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5069\/revisions"}],"predecessor-version":[{"id":5573,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/5069\/revisions\/5573"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/media\/2230"}],"wp:attachment":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5069"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}