{"id":4956,"date":"2020-07-09T09:08:45","date_gmt":"2020-07-09T01:08:45","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=4956"},"modified":"2020-07-09T09:15:01","modified_gmt":"2020-07-09T01:15:01","slug":"home-16","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=4956","title":{"rendered":"Total RNA Kit (Tissue) 2.0"},"content":{"rendered":"

[et_pb_section fb_built=”1″ specialty=”on” custom_padding_last_edited=”on|desktop” padding_top_bottom_link_2=”false” padding_left_right_link_2=”false” padding_top_2=”0px” padding_bottom_2=”0px” _builder_version=”4.0.7″ custom_padding=”0px||0px||false|false” custom_padding_tablet=”” custom_padding_phone=””][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_menu menu_id=”52″ active_link_color=”#000000″ _builder_version=”4.0.7″ menu_font=”|600|||||||” menu_text_color=”#000000″ menu_font_size=”15px” width=”100%” custom_padding=”|||0px||”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ specialty_columns=”3″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_row_inner _builder_version=”4.0.7″][et_pb_column_inner saved_specialty_column_type=”3_4″ _builder_version=”4.0.7″][et_pb_text _builder_version=”4.0.7″]<\/p>\n

\n
\n

Total RNA Kit (Tissue) 2.0<\/h1>\n
\n
\n
\n
\n
\n
\n
\n
\n
\n
Description<\/span><\/strong><\/div>\n<\/div>\n
\n
\n
\n

The Total RNA Kit (Tissue) 2.0 is designed specifically for purifying total RNA from fresh animal tissue or paraffin-embedded tissue. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.<\/p>\n

Features<\/strong>
\u2022 Format: spin column (centrifuge)
\u2022 Yield: Up to 30 \u03bcg<\/p>\n

Sample<\/strong>
30 mg of fresh animal tissue, 25 mg of paraffin-embedded tissue<\/p>\n

Applications<\/strong>
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n
\n
\n
\n

Specification<\/strong><\/p>\n

Store at<\/p>\n

\"\"<\/p>\n

# FYG307-100\u00a0<\/span><\/strong>(100 preps)<\/p>\n\n\n\n\n\n\n\n\n\n
TR Buffer<\/td>\n45 ml<\/td>\n<\/tr>\n
W1 Buffer<\/td>\n45 ml<\/td>\n<\/tr>\n
W2 Buffer<\/td>\n15 ml<\/td>\n<\/tr>\n
Elution Buffer<\/td>\n10 ml<\/td>\n<\/tr>\n
DNase Incubation Buffer<\/td>\n10 ml<\/td>\n<\/tr>\n
RZ Column<\/td>\n100 pcs<\/td>\n<\/tr>\n
Collection Tube<\/td>\n100 pcs<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

# FYG307-300\u00a0<\/span><\/strong>(300 preps)<\/p>\n\n\n\n\n\n\n\n\n\n
TR Buffer<\/td>\n125 ml<\/td>\n<\/tr>\n
W1 Buffer<\/td>\n125 ml<\/td>\n<\/tr>\n
W2 Buffer<\/td>\n25 ml x2<\/td>\n<\/tr>\n
Elution Buffer<\/td>\n30 ml<\/td>\n<\/tr>\n
DNase Incubation Buffer<\/td>\n30 ml<\/td>\n<\/tr>\n
RZ Column<\/td>\n300 pcs<\/td>\n<\/tr>\n
Collection Tube<\/td>\n300 pcs<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n
Manual<\/td>\n<\/tr>\n
\u00a0\u00a0<\/a>\"\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/article>\n<\/div>\n
\n
\n
<\/div>\n<\/div>\n<\/div>\n

[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.0.7″ min_height=”157px”][\/et_pb_section][et_pb_section fb_built=”1″ fullwidth=”on” disabled_on=”on|on|on” _builder_version=”4.0.7″ disabled=”on”][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Total RNA Kit (Tissue) 2.0 Description The Total RNA Ki […]<\/p>\n","protected":false},"author":26,"featured_media":2230,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-4956","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/4956","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4956"}],"version-history":[{"count":4,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/4956\/revisions"}],"predecessor-version":[{"id":4964,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/4956\/revisions\/4964"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/media\/2230"}],"wp:attachment":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4956"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}