EtB\u201dOut\u201d Nucleic Acid Staining Solution aims to replace traditional EtBr (ethidium bromide) in performing nucleic acid detection in agarose gels. EtBr has long been known as a strong mutagen, however EtB\u201dOut\u201d causes only neglectable mutations in the Ames test.<\/p>\n<\/div>\n
The sensitivity of EtB\u201dOut\u201d is identical to that of EtBr. Under UV light, EtB\u201dOut\u201d emits green fluorescence when bound to DNA or RNA. EtB\u201dOut\u201d can be excited at 290 nm and 490 nm. The fluorescence emission peak of EtB\u201dOut\u201d when it bound to DNA is at 537 nm.<\/p>\n<\/div>\n<\/div>\n
- \n
- Economic :<\/strong>
use only 5 \u03bcl in 100 ml of agarose gel<\/li>\n<\/ul>\n- \n
- Sensitive :<\/strong>
sensitivity is comparable and even better than EtBr, DNA concentration as low as 5 ng can be detected<\/li>\n<\/ul>\n- \n
- Safe :<\/strong>
non-mutagenic, non-toxic, non-carcinogenic<\/li>\n<\/ul>\n- \n
- Green :<\/strong>
no hazardous waste<\/li>\n<\/ul>\n<\/div>\n<\/div>\nApplications<\/strong><\/div>\n\n\nNucleic acids detection (dsDNA and ssRNA) within agarose gel after electrophoresis under UV and blue light illumination<\/p>\n<\/div>\n<\/div>\n
Quality Assurance<\/strong><\/div>\n\n\nThe quality of the EtB\u201dOut\u201d Nucleic Acid Staining Solution is tested on a lot-to-lot basis. Nucleic acids are extracted from tissues and serial diluted. Electrophoresis is performed and the agarose gel is stained with EtB\u201dOut\u201d Nucleic Acid Staining Solution to ensure the performance.<\/p>\n<\/div>\n<\/div>\n
Excitation and Emission Wavelength<\/div>\n\n<\/p>\n
![\"\"](\"https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2021\/12\/YD010-wave-length-1.png\")
<\/h1>\n<\/div>\nResults<\/strong><\/div>\n\n![\"\"](\"https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/etb-out-data.jpg\")
<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\nSpecification<\/div>\n\nStore at<\/span><\/p>\n
![\"\"](\"https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/RT-300x222.png\")
<\/p>\n# FYD010-200P<\/b>\u00a0(200 preps)<\/span><\/p>\n
\n\n\n
\n EtB\u201dOut\u201d Nucleic Acid Staining Solution 2.0 (20,000\u00d7)<\/td>\n 1 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n \n\n
\n Manual<\/td>\n<\/tr>\n \n \u00a0\u00a0<\/a><\/p>\n
- Green :<\/strong>
- Safe :<\/strong>
- Sensitive :<\/strong>
![\"\"](\"https:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\")
<\/a><\/p><\/blockquote>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n