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Description<\/span><\/strong><\/div>\n<\/div>\n
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The\u00a0Pre-stained Sharp Protein Marker III<\/strong>\u00a0is a super broad range,\u00a03-color pre-stained<\/strong>\u00a0recombinant protein standards consist of\u00a012<\/strong>proteins from\u00a010 kDa<\/strong>\u00a0to\u00a0315 kDa<\/strong>. The marker mix is pre-denatured and stabilized in loading buffer, so no additional boiling is necessary before loading onto SDS-PAGE gels.<\/div>\n<\/div>\n
Storage<\/div>\n
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Store the protein ladder for up to\u00a0three months at 4\u00b0C<\/strong>\u00a0or for\u00a0one year at -20\u00b0C<\/strong>.<\/div>\n
\u00a0<\/div>\n<\/div>\n
Features<\/strong><\/div>\n
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- Crystal clear:<\/strong>\u00a0Sharp bands and stable colors<\/li>\n
- Multicolor:<\/strong>\u00a0easy to track on gel<\/li>\n
- Ready-to-load:<\/strong>\u00a0no need to boil<\/li>\n
- Excellent Western transfer<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Applications<\/strong><\/div>\n\n\n- \n
- Monitoring protein migration during SDS-PAGE<\/li>\n
- Monitoring protein transfer onto membranes during Western blotting<\/li>\n
- Approximate sizing of proteins on SDS-PAGE gels<\/li>\n
- Acrylamide gels and Western blots<\/li>\n<\/ul>\n<\/div>\n<\/div>\nNotes<\/strong><\/div>\n\n\n
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- The ladder contains\u00a0two<\/strong>\u00a0green reference bands at\u00a0~50 kDa<\/strong>\u00a0and\u00a010 kDa<\/strong>\u00a0and one orange reference band of\u00a070 kDa<\/strong>.<\/li>\n
- Avoid multiple freeze\/thaw cycles.<\/li>\n
- Lot-to-lot variation of the apparent molecular weight of pre-stained proteins is <10% depending on the molecular weight.<\/li>\n
- Western blotting of large (>100 kDa) proteins may need longer transfer times or higher transfer voltages for efficient transfer.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Quality Control<\/strong><\/div>\n\n5 \u03bcl of\u00a0Pre-stained Sharp Protein Marker III<\/strong>\u00a0provide expected numbers of bands of equal intensity in SDS-PAGE (Tris-glycine buffer) and after electrotransfer onto a PVDF membrane.<\/div>\n<\/div>\n\u00a0<\/div>\nRecommendation for Loading<\/strong><\/div>\n\n\n- \n
- Apply\u00a05 \u03bcl\/well<\/strong>\u00a0on mini gels and\u00a010 ~ 15 \u03bcl\/well<\/strong>\u00a0on large gels<\/li>\n
- For blots, apply\u00a01 ~ 3 \u03bcl\/well<\/strong>\u00a0on mini gels and\u00a02 ~ 6 \u03bcl\/well<\/strong>\u00a0on large gels<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Results<\/strong><\/div>\n\n\u00a0
![\"\"](\"http:\/\/www.yeastern.com\/img\/upload\/image\/sharpIII.png\")
\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0![\"\"](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2018\/05\/Sharp-Protein-Marker-III-data.jpg\")
<\/p>\n<\/div>\n<\/div>\n<\/div>\n\u00a0<\/div>\n<\/div>\n\u00a0<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\u00a0<\/div>\nSpecification
Store at![\"\"](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-300x222.png\")
<\/p>\n<\/div>\n\n# FYP006-500UL<\/b><\/p>\n
\n - For blots, apply\u00a01 ~ 3 \u03bcl\/well<\/strong>\u00a0on mini gels and\u00a02 ~ 6 \u03bcl\/well<\/strong>\u00a0on large gels<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
- The ladder contains\u00a0two<\/strong>\u00a0green reference bands at\u00a0~50 kDa<\/strong>\u00a0and\u00a010 kDa<\/strong>\u00a0and one orange reference band of\u00a070 kDa<\/strong>.<\/li>\n
- Multicolor:<\/strong>\u00a0easy to track on gel<\/li>\n
![\"\"](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\")
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