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Description<\/span><\/strong><\/div>\n<\/div>\n
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Yeastern Biotech offers nucleotides with high purity for use in PCR, RT-PCR, RT assay, DNA labeling reactions and sequencing\/cycle sequencing analysis.<\/p>\n<\/div>\n<\/div>\n
Features<\/strong><\/div>\n
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- Greater than 99% purity confirmed by HPLC.<\/li>\n
- Free of trace contaminating nucleotides.<\/li>\n
- Free of endo- and exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.<\/li>\n
- Highly stable \u2013 the neutral pH of the nucleotide solutions ensures stability during long-term storage:<\/li>\n
- stable for years at -20\u00b0C,<\/li>\n
- stable after multiple freeze-thaw cycles,<\/li>\n
- 90-95% of dNTPs remain in triphosphate form after 7 weeks at room temperature,<\/li>\n
- 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94\u00b0C; 3 mins at 72\u00b0C).<\/li>\n
- Application tested in standard PCR, high fidelity PCR, long-range PCR (40 kb), cDNA synthesis, RT-PCR, and real-time PCR.<\/li>\n<\/ul>\n<\/div>\n<\/div>\nApplications<\/strong><\/div>\n\n\n
Yeastern Biotech dNTPs can be used in all molecular biology applications including standard PCR, high fidelity, long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, real-time PCR, RDA, MDA, DNA labeling and DNA sequencing.<\/p>\n<\/div>\n<\/div>\n
Quality Control<\/strong><\/div>\n\n\n- \n
- dCTP, dATP, dGTP and dTTP are all in the form of sodium salt (pH8.3); >99% dCTP (HPLC), <0.9% dCDP.<\/li>\n
- Greater than 99% purity of each component confirmed by HPLC. Functionally tested in PCR with Taq and Pfu DNA Polymerases. The absence of endo-, exodeoxyribonuclease, ribonuclease and nicking activities confirmed by appropriate tests.<\/li>\n<\/ul>\n
![\"\"](\"http:\/\/www.yeastern.com\/img\/upload\/P1030728.png\")
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![\"\"](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/07\/20C-300x222.png\")
<\/p>\n# FYT013-200UL<\/b><\/span><\/p>\n
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![\"\"](\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\")
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