{"id":4689,"date":"2020-07-07T15:51:09","date_gmt":"2020-07-07T07:51:09","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=4689"},"modified":"2020-07-07T17:39:35","modified_gmt":"2020-07-07T09:39:35","slug":"%e5%b7%a6%e6%ac%84-2","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=4689","title":{"rendered":"Total RNA Kit (Plant)2.0"},"content":{"rendered":"

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Description<\/strong>
The Total RNA Kit (Plant) 2.0 is designed specifically for purifying total RNA from plant tissue or dry plant tissue. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber\u00a0matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.<\/p>\n<\/div>\n

Features<\/strong>
\u2022 Format: spin column (centrifuge)
\u2022 Yield: Up to 30 \u03bcg<\/p>\n

Sample<\/strong>
100 mg of plant Tissue, 25 mg of dry plant Tissue<\/p>\n

Applications<\/strong>
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis<\/p>\n<\/div>\n<\/div>\n

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Specification<\/p>\n

Store at<\/p>\n

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# FYG308-100 <\/strong>(100 preps)<\/p>\n\n\n\n\n\n\n\n\n
PR Buffe<\/td>\n110 ml<\/td>\n<\/tr>\n
W1 Buffer<\/td>\n45 ml<\/td>\n<\/tr>\n
W2 Buffer<\/td>\n15 ml<\/td>\n<\/tr>\n
Elution Buffer<\/td>\n10 ml<\/td>\n<\/tr>\n
RZ Column<\/td>\n100 pcs<\/td>\n<\/tr>\n
Collection Tube<\/td>\n100 pcs<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

# FYG308-300 <\/strong>(300 preps)<\/p>\n\n\n\n\n\n\n\n\n
PR Buffer<\/td>\n105 ml x3<\/td>\n<\/tr>\n
W1 Buffer<\/td>\n125 ml<\/td>\n<\/tr>\n
W2 Buffer<\/td>\n25 ml x2<\/td>\n<\/tr>\n
Elution Buffer<\/td>\n30 ml<\/td>\n<\/tr>\n
RZ Column<\/td>\n300 pcs<\/td>\n<\/tr>\n
Collection Tube<\/td>\n300 pcs<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n
Manual<\/td>\n<\/tr>\n
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Description The Total RNA Kit (Plant) 2.0 is designed s […]<\/p>\n","protected":false},"author":26,"featured_media":2230,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-4689","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/4689","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4689"}],"version-history":[{"count":6,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/4689\/revisions"}],"predecessor-version":[{"id":4791,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/4689\/revisions\/4791"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/media\/2230"}],"wp:attachment":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4689"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}