{"id":4528,"date":"2020-06-23T17:01:13","date_gmt":"2020-06-23T09:01:13","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=4528"},"modified":"2020-07-21T11:47:41","modified_gmt":"2020-07-21T03:47:41","slug":"xpress-oin-1-dna-polymerase-premix-2x-w-violet-dye","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=4528","title":{"rendered":"Xpress O\u2019in 1 DNA Polymerase Premix (2x, w\/ violet dye)"},"content":{"rendered":"

[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”3.22″][et_pb_row column_structure=”1_4,3_4″ use_custom_gutter=”on” gutter_width=”1″ _builder_version=”4.0.7″ width=”85%” max_width=”2560px” module_alignment=”center” custom_padding=”30px|0px|0px|0px|false|false”][et_pb_column type=”1_4″ _builder_version=”4.0.7″][et_pb_menu menu_id=”52″ menu_style=”centered” active_link_color=”#000000″ _builder_version=”4.0.7″ menu_font=”|600|||||||” menu_text_color=”#000000″ menu_font_size=”16px” width=”76%” custom_margin=”||||false|false” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop” custom_padding=”|31px||||”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.0.7″][et_pb_post_title meta=”off” featured_image=”off” admin_label=”Post Title” _builder_version=”4.0.7″][\/et_pb_post_title][et_pb_text _builder_version=”4.0.7″ custom_padding=”||||false|false”][\/et_pb_text][et_pb_toggle title=”Product overview” _builder_version=”4.0.7″ title_level=”h4″ title_font=”|800|||||||” closed_title_font=”|600|||||||”]<\/p>\n

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Description<\/strong><\/h6>\n
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Xress O’ in 1 DNA Polymerase Premix is a master mix including fast DNA polymerase, reaction buffer, and dye in 1 tube. It saves the time for preparing the master mix and reduces the risk of contamination from multiple pipetting steps. The fast taq in Xpress O\u2019in 1\u00a0 DNA Polymerase Premixe is developed for fast PCR especially for fast screening and genotyping. The highest elongation speed of the taq in this kit is 2 kb\/s and the sensitivity is 100 fg.<\/p>\n<\/div>\n

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Optimal PCR conditions, including template and primer concentrations and PCR program, for the gene of interest, should be determined experimentally by the investigator from case to case.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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  • Convenient: Taq DNA polymerase in a ready-to-use mix with dye<\/li>\n
  • Reproducible: lower contamination and pipetting error risk<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
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    • Ready to use<\/li>\n
    • Master mix<\/li>\n
    • Suitable for economic screening<\/li>\n
    • High throughput PCR<\/li>\n
    • Routine PCR with high reproducibility<\/li>\n
    • Generation of PCR products for TA cloning<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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      Specifications<\/strong><\/h6>\n
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      # FYT211-100P<\/b>\u00a0(100 preps)\u00a0<\/span><\/p>\n

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      Xpress O\u2019\u00a0in 1 DNA Polymerase Premix (2\u00d7)<\/strong>*\u00a0 with Violet Dye<\/p>\n

      * <\/b>2\u00d7 O\u2019in 1 DNA Polymerase Premix<\/span><\/p>\n

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      		1.25 ml x 2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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      Unit Definition<\/strong><\/div>\n
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      One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into an acid-insoluble material in 30 minutes at 72\u00b0C.<\/p>\n<\/div>\n<\/div>\n

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      Quality Control<\/strong><\/p>\n<\/div>\n<\/div>\n

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      • Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb \/Hind<\/em>\u00a0III DNA with 5 units of HiFi DNA Polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C.<\/li>\n
      • The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
        Patterns\/Disclaimer<\/strong><\/div>\n
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        Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because the purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.<\/p>\n

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        [\/et_pb_toggle][et_pb_image src=”http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/06\/Xpress-DNA-Polymerase-Premix.png” admin_label=”Image” _builder_version=”4.0.7″ custom_margin=”55px|||||”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

        Description Xress O’ in 1 DNA Polymerase Premix is a ma […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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        Description<\/strong><\/div>\r\n<\/div>\r\n
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        O\u2019in 1 DNA Polymerase Premix is an economical and ready-to-use premix, containing YEAtaq DNA Polymerase (# FYT001-500U<\/strong>), dNTP and all other reagents necessary for PCR, except DNA template and primers. It saves the time for preparing the master mix and reduces the risk of contamination from multiple pipetting steps. O\u2019in 1 2\u00d7 DNA Polymerase Premixes are also available with different non-interfering dyes for applications when loading dyes are desired. Users can choose from 5 different colors, including red, orange (mix of red and orange), violet, green (mix of blue and orange), and blue, depending on the preferred dye locations on the gel.<\/p>\r\n<\/div>\r\n

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        Optimal PCR conditions, including template and primer concentrations and PCR program, for the gene of interest, should be determined experimentally by the investigator from case to case.<\/p>\r\n<\/div>\r\n<\/div>\r\n

        Features<\/strong><\/div>\r\n
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