{"id":3953,"date":"2020-02-13T11:26:51","date_gmt":"2020-02-13T03:26:51","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=3953"},"modified":"2023-04-07T13:31:25","modified_gmt":"2023-04-07T05:31:25","slug":"ecos-sonic-competent-cells-bl21de3","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=3953","title":{"rendered":"ECOS\u2122 Sonic\u2122 Competent Cells [BL21(DE3) Derived]"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.0.7″][et_pb_row column_structure=”1_4,3_4″ use_custom_gutter=”on” gutter_width=”1″ _builder_version=”4.0.7″ width=”85%” max_width=”2560px” module_alignment=”center” custom_padding=”30px|0px|0px|0px|false|false”][et_pb_column type=”1_4″ _builder_version=”4.0.7″][et_pb_menu menu_id=”52″ menu_style=”centered” active_link_color=”#000000″ _builder_version=”4.0.7″ menu_font=”|600|||||||” menu_text_color=”#000000″ menu_font_size=”16px” width=”69.8%” custom_margin=”||88px||false|false” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.0.7″][et_pb_post_title meta=”off” featured_image=”off” _builder_version=”4.0.7″ title_font=”|700|||||||”][\/et_pb_post_title][et_pb_text _builder_version=”4.0.6″ custom_padding=”||||false|false”]<\/p>\n

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[\/et_pb_text][et_pb_toggle title=”Product overview” _builder_version=”4.0.6″ title_level=”h4″ title_font=”|800|||||||” closed_title_font=”|600|||||||”]<\/p>\n

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Description<\/strong><\/h6>\n<\/div>\n
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We have taken it upon ourselves to continue the production of\u00a0Acella\u2122 cells<\/strong> previously from Edge Biosystems Inc., which we have now renamed as ECOS\u2122 Sonic\u2122<\/strong><\/p>\n

Sonic\u2122\u00a0Competent Cells\u00a0allow you to clone and express in one novel strain. Also available with the pLysS plasmid, the\u00a0Sonic\u2122 strain is a BL21-derivative that features complete deletions of the endA<\/em> and recA<\/em> genes, eliminating degradation and recombination of DNA.<\/p>\n

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For optimal results, recommended for use in combination with Yeastern’s proprietary T&A\u2122 expression vector<\/strong><\/a>, which will combine transformation, cloning and protein expression into one single step.<\/div>\n
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Features<\/strong><\/h6>\n

Fast<\/span><\/strong>: Only 4-6hrs culture time required to isolates plasmid DNA<\/p>\n

Stable<\/span><\/strong>: Lack of omp<\/i>T and Ion proteases to stabilize synthesized protein; deletion of end<\/i>A and rec<\/i>A eliminates DNA degradation and recombination<\/p>\n

High efficiency and protein yield<\/strong><\/span>: 1 x 108<\/sup>\u00a0cfu\/ug<\/p>\n

Simple to use<\/strong><\/span>: clone and express with the same strain, no more sub-cloning required!<\/p>\n

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Applications<\/strong><\/h6>\n
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