{"id":2462,"date":"2017-08-04T11:54:38","date_gmt":"2017-08-04T03:54:38","guid":{"rendered":"http:\/\/si.secda.info\/yb_md\/?page_id=2462"},"modified":"2020-08-19T15:05:30","modified_gmt":"2020-08-19T07:05:30","slug":"protein-expression","status":"publish","type":"page","link":"https:\/\/si.secda.info\/yb_md\/?page_id=2462","title":{"rendered":"Vectors &#038; Protein Expression"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; specialty=&#8221;on&#8221; _builder_version=&#8221;4.0.7&#8243;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_menu menu_id=&#8221;52&#8243; menu_style=&#8221;centered&#8221; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.0.7&#8243; menu_font=&#8221;|600|||||||&#8221; menu_text_color=&#8221;#000000&#8243; menu_font_size=&#8221;16px&#8221; custom_margin=&#8221;||||false|false&#8221; custom_margin_tablet=&#8221;&#8221; custom_margin_phone=&#8221;&#8221; custom_margin_last_edited=&#8221;on|desktop&#8221;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; specialty_columns=&#8221;3&#8243; _builder_version=&#8221;3.25&#8243; custom_padding=&#8221;|||&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_row_inner _builder_version=&#8221;4.0.7&#8243;][et_pb_column_inner saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_post_title meta=&#8221;off&#8221; featured_image=&#8221;off&#8221; _builder_version=&#8221;4.0.7&#8243; title_font_size=&#8221;40px&#8221;][\/et_pb_post_title][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=&#8221;4.0.7&#8243; min_height=&#8221;65px&#8221; custom_margin=&#8221;-38px||-38px||false|false&#8221;][et_pb_column_inner saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.7&#8243; text_font=&#8221;|600|||||||&#8221;]<\/p>\n<h2>in Prokaryotes (<em>E.coli\u00a0<\/em>)<\/h2>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.0.7&#8243; custom_margin=&#8221;-21px||46px|||&#8221; custom_padding=&#8221;25px||0px|||&#8221; locked=&#8221;off&#8221;][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_font=&#8221;||||||||&#8221; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; text_line_height=&#8221;1em&#8221; header_3_font=&#8221;||||||||&#8221; header_3_letter_spacing=&#8221;1px&#8221; header_3_line_height=&#8221;1.8em&#8221; width=&#8221;100%&#8221; min_height=&#8221;520px&#8221; custom_margin=&#8221;-7px||24px|0px|false|false&#8221; custom_padding=&#8221;2px||64px|0px|false|false&#8221; link_option_url=&#8221;?page_id=1384&#8243; hover_enabled=&#8221;0&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221; inline_fonts=&#8221;Verdana,Times New Roman&#8221;]<\/p>\n<h2 style=\"text-align: center;\"><strong><\/strong><\/h2>\n<h3 style=\"text-align: center;\"><strong>T&amp;A\u2122 Cloning Kit<\/strong><\/h3>\n<p><strong><\/strong><\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/ta-cloning-kit.png\" width=\"335\" height=\"236\" alt=\"\" class=\"wp-image-5513 alignnone size-full\" \/><\/p>\n<p><strong><\/strong><\/p>\n<p><span><strong><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><b>Easy Operation &amp; Rapid Ligation<\/b><\/p>\n<p style=\"text-align: center;\"><span><strong><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong>The best value choice for your routine<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong> cloning !<\/strong><\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column_inner][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; text_line_height=&#8221;1em&#8221; header_3_font=&#8221;||||||||&#8221; header_3_letter_spacing=&#8221;1px&#8221; header_3_line_height=&#8221;1.8em&#8221; width=&#8221;100%&#8221; min_height=&#8221;546px&#8221; custom_margin=&#8221;-8px||0px|||&#8221; custom_padding=&#8221;0px||0px|||&#8221; link_option_url=&#8221;?page_id=1949&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221;]<\/p>\n<h3 style=\"text-align: center;\"><strong><\/strong><\/h3>\n<h3 style=\"text-align: center;\"><strong>T&amp;A\u2122 Expression Kit<\/strong><\/h3>\n<p><strong><\/strong><\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/ta-expression.jpg\" width=\"315\" height=\"272\" alt=\"\" class=\"wp-image-5735 alignnone size-full\" \/><\/p>\n<p style=\"text-align: center;\"><b>Fewer Cloning Times<\/b><\/p>\n<p style=\"text-align: center;\"><strong><span><\/span><\/strong><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong>Cloning for proteins expression in <\/strong><strong>one\u00a0<\/strong><\/span><strong style=\"font-size: medium;\">step !<\/strong><\/p>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=&#8221;4.0.7&#8243; min_height=&#8221;65px&#8221; custom_margin=&#8221;-14px||-38px||false|false&#8221;][et_pb_column_inner saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.7&#8243; text_font=&#8221;|600|||||||&#8221;]<\/p>\n<h2>in Eukaryotes (Yeast)<\/h2>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.0.6&#8243; custom_margin=&#8221;-21px||-31px|||&#8221; custom_padding=&#8221;||24px|||&#8221; locked=&#8221;off&#8221;][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; text_line_height=&#8221;1em&#8221; header_3_letter_spacing=&#8221;1px&#8221; header_3_line_height=&#8221;1.8em&#8221; width=&#8221;100%&#8221; min_height=&#8221;566px&#8221; custom_margin=&#8221;-7px||59px|||&#8221; custom_padding=&#8221;2px||0px||false|false&#8221; link_option_url=&#8221;?page_id=1388&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221; inline_fonts=&#8221;Verdana&#8221;]<\/p>\n<h3 style=\"text-align: center;\"><strong><span style=\"color: #008000;\"><\/span><\/strong><\/h3>\n<h3 style=\"text-align: center;\"><strong><span style=\"color: #008000;\">SCOS<\/span>\u00a0<\/strong><strong style=\"font-size: 22px;\">Transformation Kit<\/strong><\/h3>\n<p><strong><sup><\/sup><\/strong><\/p>\n<h5 style=\"text-align: center;\"><strong><sup>&#8220;<em><span style=\"color: #008000;\">S<\/span>accharomyces <span color=\"#008000\" style=\"color: #008000;\">C<\/span>erevisiae<\/em>\u00a0<span color=\"#008000\" style=\"color: #008000;\">O<\/span>ne-<span color=\"#008000\" style=\"color: #008000;\">S<\/span>tep&#8221;<\/sup><\/strong><\/h5>\n<p style=\"text-align: center;\"><strong><sup><span><\/span><\/sup><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><sup><span><\/span><\/sup><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><sup><span><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/SCOS.png\" width=\"1360\" height=\"1018\" alt=\"\" class=\"wp-image-5452 alignnone size-full\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/SCOS.png 1360w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/SCOS-1280x958.png 1280w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/SCOS-980x734.png 980w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/SCOS-480x359.png 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1360px, 100vw\" \/>\u00a0<\/span><\/sup><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><sup><span>Simple &amp;<\/span><\/sup><\/strong><strong><sup><span>\u00a0Fast <\/span><\/sup><\/strong><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong><sup>One-step, one-tube\u00a0<\/sup><\/strong><strong><sup>method for yeast transformation !<\/sup><\/strong><\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column_inner][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;3_4&#8243; _builder_version=&#8221;4.0.7&#8243;][et_pb_text _builder_version=&#8221;4.0.6&#8243; text_text_color=&#8221;#158701&#8243; text_font_size=&#8221;18px&#8221; text_line_height=&#8221;1em&#8221; header_3_letter_spacing=&#8221;1px&#8221; header_3_line_height=&#8221;1.8em&#8221; header_4_text_color=&#8221;#158701&#8243; header_4_font_size=&#8221;16px&#8221; width=&#8221;100%&#8221; custom_margin=&#8221;-7px||3px||false|false&#8221; custom_padding=&#8221;2px||34px||false|false&#8221; link_option_url=&#8221;?page_id=1390&#8243; border_radii=&#8221;on|10px|10px|10px|10px&#8221; border_width_all=&#8221;1px&#8221; border_color_all=&#8221;rgba(124,218,36,0.5)&#8221;]<\/p>\n<h3 style=\"text-align: center;\"><strong><span style=\"color: #008000;\"><\/span><\/strong><\/h3>\n<h3 style=\"text-align: center;\"><strong><span style=\"color: #008000;\">YLEX<\/span>\u00a0<\/strong><span style=\"font-size: x-large;\"><strong>Yeast Expression Kit<\/strong><\/span><\/h3>\n<p style=\"text-align: center;\"><strong><sup><\/sup><\/strong><\/p>\n<h5 style=\"text-align: center;\"><strong><sup><em><span>&#8220;<span style=\"color: #008000;\">Y<\/span><\/span>arrowia <span color=\"#008000\" style=\"color: #008000;\">L<\/span>ipolytica<\/em> <span style=\"color: #008000;\">EX<\/span>pression&#8221;<\/sup><\/strong><\/h5>\n<p><strong><sup><\/sup><\/strong><\/p>\n<p><strong><sup><\/sup><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><sup><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/YLEX.png\" width=\"1414\" height=\"1082\" alt=\"\" class=\"wp-image-5453 alignnone size-full\" srcset=\"http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/YLEX.png 1414w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/YLEX-1280x979.png 1280w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/YLEX-980x750.png 980w, http:\/\/si.secda.info\/yb_md\/wp-content\/uploads\/2020\/07\/YLEX-480x367.png 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1414px, 100vw\" \/><\/sup><\/strong><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: large;\"><strong>\u00a0<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: large;\"><strong><sup>Safe &amp; Less Hyperglycosylation<\/sup><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: medium;\"><strong><sup>Classified as GRAS by the US FDA<\/sup><\/strong><\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>in Prokaryotes (E.coli\u00a0) T&amp;A\u2122 Cloning Kit Easy Oper [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"Prokaryote (E.coli)\r\n<ul id=\"menu-prokaryote-e-coli\" class=\"menu\">\r\n \t<li id=\"menu-item-2470\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2470\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1949\">T&A Expression Kit<\/a><\/li>\r\n<\/ul>\r\nEukaryote(Yeast)\r\n<ul id=\"menu-eukaryoteyeast\" class=\"menu\">\r\n \t<li id=\"menu-item-1452\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1452\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1388\">SCOS Transformation Kit<\/a><\/li>\r\n \t<li id=\"menu-item-1451\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1451\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1390\">YLEX Yeast Expression Kit<\/a><\/li>\r\n \t<li id=\"menu-item-1450\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-1450\"><a href=\"http:\/\/si.secda.info\/yb_md\/?page_id=1392\">YLOS Yeast Transformation Kit<\/a><\/li>\r\n<\/ul>","_et_gb_content_width":"","footnotes":""},"class_list":["post-2462","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/2462","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2462"}],"version-history":[{"count":36,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/2462\/revisions"}],"predecessor-version":[{"id":6099,"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=\/wp\/v2\/pages\/2462\/revisions\/6099"}],"wp:attachment":[{"href":"https:\/\/si.secda.info\/yb_md\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2462"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}