O’in 1 DNA Polymerase Premix II

O’in1 DNA Polymerase Premix II (2X) w/dye

O’in1 DNA Polymerase Premix II (2X) w/dye

O’in1 DNA Polymerase Premix II is an economical and ready-to-use premix, containing a high-sensitivity and high-yield YEAtaq DNA Polymerase, dNTP and all other reagents necessary for PCR, except DNA template and primers. It saves the time for preparing the master mix and reduces the risk of contamination from multiple pipetting steps. O’ in 1 2X DNA Polymerase Premix II is also available with non-interfering dye for applications when loading dye is desired.

Optimal PCR conditions, including template, primer concentrations and PCR program, for gene of interest should be determined experimentally by the investigator from case to case.

Features

  • Convenient: Taq DNA polymerase in a ready-to-use mix with or without dyes
  • Reproducible: lower contamination and pipetting error risk

Applications

  • Ready to use
  • Master mix
  • Suitable for economic screening
  • High throughput PCR
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72 °C

Size of Dye (in 1% agarose gel)

Blue dye: ~500 bp

Quality Control

  • Nuclease activity is not detected after incubation of 1 μg λ /Hind III DNA with 5 units of HiFi DNA Polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
  • The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR.

Patterns/Disclaimer

Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because the purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Result

Various amount of DNA fragment are tested with detect-ability as low as 100fg up to 100fg samples. 

Specification

Store at

# FYT208-200P (200 preps) 

O’ in 1 DNA Polymerase Premix II (2×)