T&A Expression Kit

Description

The key to protein expression is an expression vector, Yeastern Biotech’s T&ATM Expression Kit (C-terminal) combine fast ligation of T-A cloning and expressing features of the T7 promoter. It offers a quick and efficient method of protein expression.

The T&A™ Expression Kit (C-terminal) (FYC201-10P) contains the T&A™ Expression Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3’-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™).

Features

  • Fast ligation, completed in only 5 minutes
  • Bacteriophage T7 promoter for high-level expression
  • C-terminal 6x His tag for detection or purification
  • High transformation efficiency
  • Fast ligation, completed in only 5 minutes
  • High transformation efficiency
  • Two types of ligation buffers provided for your convenience
  • Allow blue/white screening
  • Contain ampicillin marker for antibiotic selection
  • Include T7 promoter primer sites for convenient sequencing

Application

  • Gene construction and protein expression.

 

 

 

 

Map & Sequence Reference Points

 

 

DNA Sequence of Multiple Cloning Sites (MCS)

#FYC201-10P (10 preps)

T&ATM Expression Kit  (C-terminal)

T&A Expression Vector  (C-terminal) 20 μl (25 ng/μl)
Control Insert DNA (C-terminal) 10 μl (10 ng/μl)
yT4 DNA Ligase (2U) 10 μl
10X Ligation Buffer A 50 μl
10X Ligation Buffer B 50 μl
Primer T7 promoter 50 μl (10μM)
Primer T7 terminator 50 μl (10μM)

 

#FYC211-10P (10 preps)

T&ATM Expression Vector (C-terminal)

T&A Expression Vector  (C-terminal) 20 μl (25 ng/μl)
Control Insert DNA (C-terminal) 10 μl (10 ng/μl)
Primer T7 promoter 50 μl (10μM)
Primer T7 terminator 50 μl (10μM)
Manual