T&A Expression Kit

Description

The key of protein expression is expression vector, Yeastern Biotech’s T&ATM Expression Kit (C-terminal) combine fast ligation of T-A cloning and expressing features of T7 promoter. It offers quick and efficient method of protein expression.

The T&A™ Expression Kit (C-terminal) (FYC201-10P) contains the T&A™ Expression Vector andall the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNApolymerase, which add a single terminal 3’-dA nucleotide overhang. Afterligation, the mixture can be used directly for transformation into competentcells (ECOS™). 

Features

  • Fast ligation, completed in only 5 minutes
  • Bacteriophage T7 promoter for high level expression
  • C-terminal 6x His tag for detection or purification
  • High transformation efficiency
  • Fast ligation, completed in only 5 minutes
  • High transformation efficiency
  • Two types of ligation buffers provided for your convenience
  • Allow blue/white screening
  • Contain ampicillin marker for antibiotic selection
  • Include T7 promoter primer sites for convenient sequencing

Application

Gene construction and protein expression.

Enzyme Position Enzyme Position Enzyme Position Enzyme Position Enzyme Position
AccI 2511 Bbr7I 3155 BseYI 1976 HaeIV 1392 PstI 1146
AfeI 3028 BbsI 3155 BsgI 3105 MscI 3311 PvuI 1020
AflIII 2280 BglI 1273 BsmBI 2633 NaeI 143 PvuII 2691
AhdI 1392 BglII 3334 BstZ17I 2511 NgoMIV 143 SapI 2403
AloI 302 BlpI 3525 BtgI 3308 Nli3877I 3330 ScaI 909
AlwNI 1869 Bpu10I 3175 DraIII 249 PciI 2280 StyI 3547
AvaI 3330 BsaHI 851 FspAI 3302 PfoI 2638 Tth111I 2538
BanII 173 BsaXI 300 GdiII 999 PsiI 374 XbaI 3392

#FYC201-10P (10 preps)

T&ATM Expression Kit  (C-terminal)

T&A Expression Vector  (C-terminal) 20 μl (25 ng/μl)
Control Insert DNA (C-terminal) 10 μl (10 ng/μl)
yT4 DNA Ligase (2U) 10 μl
10X Ligation Buffer A 50 μl
10X Ligation Buffer B 50 μl
Primer T7 promoter 50 μl (10μM)
Primer T7 terminator 50 μl (10μM)

 

#FYC211-10P (10 preps)

T&ATM Expression Vector (C-terminal)

T&A Expression Vector  (C-terminal) 20 μl (25 ng/μl)
Control Insert DNA (C-terminal) 10 μl (10 ng/μl)
Primer T7 promoter 50 μl (10μM)
Primer T7 terminator 50 μl (10μM)