EtB"Out" Nucleic Acid Staining Solution aims to replace traditional EtBr (ethidium bromide) in performing nucleic acid detection in agarose gels. EtBr has long been known as a strong mutagen, however EtB"Out" causes only neglectable mutations in the Ames test.
The sensitivity of EtB"Out" is identical to that of EtBr. Under UV light, EtB"Out" emits green fluorescence when bound to DNA or RNA. EtB"Out" can be excited at 309 nm, 419 nm, and 514 nm. The fluorescence emission peak of EtB"Out" when it bound to DNA is at 537 nm.
- Economic :
use only 5 μl in 100 ml of agarose gel
- Sensitive :
sensitivity is comparable and even better than EtBr, DNA concentration as low as 5 ng can be detected
- Safe :
non-mutagenic, non-toxic, non-carcinogenic
- Green :
no hazardous waste
Nucleic acids detection (dsDNA and ssRNA) within agarose gel after electrophoresis under UV illumination
The quality of the EtB"Out" Nucleic Acid Staining Solution is tested on a lot-to-lot basis. Nucleic acids is extracted from tissues and serial diluted. Electrophoresis is performed and the agarose gel is stained with EtB"Out" Nucleic Acid Staining Solution to ensure the performance.
Excitation and Emission Wavelength