Total RNA Kit (Tissue) 2.0

Description
The Total RNA Kit (Tissue) 2.0 is designed specifically for purifying total RNA from fresh animal tissue or paraffin-embedded tissue. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.

Features
• Format: spin column (centrifuge)
• Yield: Up to 30 μg

Sample
30 mg of fresh animal tissue, 25 mg of paraffin-embedded tissue

Applications
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis

Specification

Store at

# FYG307-100 (100 preps)

TR Buffer 45 ml
W1 Buffer 45 ml
W2 Buffer 15 ml
Elution Buffer 10 ml
DNase Incubation Buffer 10 ml
RZ Column 100 pcs
Collection Tube 100 pcs

# FYG307-300 (300 preps)

TR Buffer 125 ml
W1 Buffer 125 ml
W2 Buffer 25 ml x2
Elution Buffer 30 ml
DNase Incubation Buffer 30 ml
RZ Column 300 pcs
Collection Tube 300 pcs
Manual