UniversAll™ Tissue Extraction/PCR Kits
 
Description
The UniversAll™ Tissue PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from a wide range of biological samples prior to amplifying targets of interest by PCR. Genomic DNA is extracted from a small amount of samples simply by incubation in UniversAll™ Extract buffer for 10 minutes at 95 °C. The UniversAll™ Tissue PCR Kits offers a novel one-step UniversAll™ Extract buffer that eliminates the need for freezing of cells or tissues with liquid nitrogen, mechanical disruption, organic extraction, column DNA purification, or alcohol precipitation.
Features
    • Simple : 
      single-step extraction of genomic DNA to PCR. No phenol/chloroform.
    • Fast : 
      cells or tissues to PCR in 10 minutes.
  • Convenient : 
    includes our superior PCR enzymes and buffers for amplification directly from the extract for your convenience.
Applications
  • Gene cloning
  • Small or large scale PCR-based genotyping
  • Quantitative PCR real-time PCR
  • Suitable for rapid forensic and disease detection and diagnosis
Quality Control
Each lot of extraction buffer was tested for its extraction capability of standard animal and plant tissues for PCR and real-time PCR. It also has to pass the stability test before shipping.

Results

 

Specification
Store at 

 

# FYU001-100P (100 preps) 

Univers All™ Extraction Buffer 5 ml
Primer U18S-F (10 μM) 20 μl
Primer U18S-R (10 μM) 20 μl
Primer P18S-F (10 μM) 20 μl
Primer P18S-F (10 μM) 20 μl
10× PCR Reaction Buffer (with 20 mM Mg²+)     500 μl
dNTPs Mix (10 mM) 50 μl
Uti DNA Polymerase (5U/μl) 50 μl
Extraction Buffer Enhancer 250 μl

# FYU002-5ML 

Univers All™ Extraction Buffer 5 ml
Extraction Buffer Enhancer 250 μl

# FYU003-20ML 

Univers All™ Extraction Buffer for animal tissue 20 ml

# FYU004-5ML 

Univers All™ Extraction Buffer II 5 ml

# FYT007-500U (500 units) 

10× PCR Reaction Buffer (with 20 mM Mg²+) 1 ml × 2
dNTPs Mix (10 mM) 250 μl
Uti DNA Polymerase
(5 U/μl)
100 μl

# FYT077-500U (500 units) 

10× PCR Reaction Buffer (with 20 mM Mg²+) 1 ml × 2
Uti DNA Polymerase
(5 U/μl)
100 μl

# FYT101-100P (100 preps) 

RealStart DNA 1.25 ml
Polymerase  Premix (2×)