HiYield Gel PCR DNA Fragments Extraction Kit 2.0
Desciption
The HiYield Gel/PCR DNA Fragments Extraction Kit 2.0 is designed to recover or
concentrate DNA fragments (50 bp-10 kb) from agarose gels in 20 mins, PCR or
other enzymatic reactions. The unique dual purpose application and high yield
mini columns make this kit valuable. The method uses a chaotropic salt,
guanidine thiocyanante to dissolve agarose gel and denature enzymes. DNA
fragments in chaotropic salt solution bind to the glass fiber matrix of the spin
column. Following washing off contaminants, the purified DNA fragments
(Effective Binding Capacity Approx: 20 ug ) are eluted by addition of low salt
elution buffer or water. Salts, enzymes and unincorporated nucleotides are
effectively removed from reaction mixtures without phenol extraction or
alcohol precipitation
 
Features
•Recovery : Up to 95%
•Operation time : within 20 minutess
Sample
100 μl PCR Product, 300 mg of Agarose Gel
Applications
Fluorescent/radioactive sequencing, PCR, restriction enzyme digestion, DNA
labeling and ligation
Specification
Store at 

 

# FYG206-100 (100 preps)

EZ Buffer

60 ml

W1 Buffer

45 ml

W2 Buffer

15 ml

Elution Buffer

10 ml

EZ Column

100 pcs

Collection Tube

100 pcs

# FYG206-300 (300 preps)

EZ Buffer

80 ml x2

W1 Buffer

300 pcs

W2 Buffer

25 ml x2

Elution Buffer

30 ml

EZ Column

125 ml

Collection Tube

300 pcs