Direct PCR Kits for Blood/Plants
Description
YB Direct PCR Kits for Blood/Plants are formulated to perform PCR directly from whole blood or plant leaf tissues with no prior DNA extraction. The Direct PCR for Blood can process whole blood (or serum) preserved in EDTA, citrate or heparin or stored on various types of filter papers. Plant tissues even with high polyphenolic compounds are all suitable for Direct PCR for Plants. The Kits employs our especial mixes of DNA polymerases and reagents allowing higher resistance to inhibitors found in blood and plants, respectively.

The Blood Direct PCR kit has been optimized to give excellent results with blood originated from many animals. The recommended blood concentration is 1−10 % and the recommended plant tissue size is about 2 mm². YB Direct PCR Kits includes a pair of universal control primers that are compatible with a number of mammalian and plant species, respectively .

Features
  • No need to purify DNA prior to PCR
  • Minimizes the risk of DNA contamination during reagent handling
  • Minute sample material required
Applications
  • Molecular diagnostic test
  • Forensic DNA Analysis
  • Identity testing
  • Multiplex PCR/SNP detection/PCR-RFLP
  • DNA sequencing/Cloning
  • Laboratory automatic PCR
  • Quantitative PCR
  • Genotyping
Quality Control
The performances of YB Direct PCR kits are tested in PCR reaction using human blood and with universal primer sets. The sensitivities of the kits are verified by the detection of specific sizes of products by DNA agarose gel and DNA sequencing.

Results

Specification
Store at 

 

Direct PCR Kit for Blood
# FYT302-100P
 (100 preps) 

2× Premix 1.25 ml
HiFi DNA Polymerase 50 μl
Enhancer P1 200 μl
Enhancer P2 200 μl
Universal Primer Set 100 μl

Direct PCR Kit for Plants
# FYT303-100P
 (100 preps) 

2× Premix 1.25 ml
HiFi DNA Polymerase 50 μl
Enhancer P1 200 μl
Enhancer P2 200 μl
Universal Primer Set 100 μl