Deoxynucleotides (dNTPs)
Description
Yeastern Biotech offers nucleotides with high purity for use in PCR, RT-PCR, RT assay, DNA labeling reactions and sequencing/cycle sequencing analysis.

Features
  • Greater than 99% purity confirmed by HPLC.
  • Free of trace contaminating nucleotides.
  • Free of endo- and exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.
  • Highly stable – the neutral pH of the nucleotide solutions ensures stability during long-term storage:
  • stable for years at -20°C,
  • stable after multiple freeze-thaw cycles,
  • 90-95% of dNTPs remain in triphosphate form after 7 weeks at room temperature,
  • 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 mins at 72°C).
  • Application tested in standard PCR, high fidelity PCR, long range PCR (40 kb), cDNA synthesis, RT-PCR, and real-time PCR.
Applications
Yeastern Biotech dNTPs can be used in all molecular biology applications including standard PCR, high fidelity, long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, real-time PCR, RDA, MDA, DNA labeling and DNA sequencing.

Quality Control
  • dCTP, dATP, dGTP and dTTP are all in the form of sodium salt (pH8.3); >99% dCTP (HPLC), <0.9% dCDP.
  • Greater than 99% purity of each component confirmed by HPLC. Functionally tested in PCR with Taq and Pfu DNA Polymerases. The absence of endo-, exodeoxyribonuclease, ribonuclease and nicking activities confirmed by appropriate tests.

Specification
Store at 

 

# FYT013-200UL 

dNTPs (10 mM) 200 μl

# FYT014-100UL 

dCTP (100 mM) 100 μl

# FYT015-100UL 

dATP (100 mM) 100 μl

# FYT016-100UL 

dGTP (100 mM) 100 μl

# FYT017-100UL 

dTTP (100 mM) 100 μl