EZtime Real-Time PCR Premix
Description
The EZtime Real-Time PCR Premix (# FYT103-100P or # FYT104-100P) is a ready-to-use, 2× concentrated PCR premix, including Hotstart Taq, SYBR Green I, optimized reaction buffer and nucleotides (# FYT104-100P with ROX) for running quantitative real-time PCR, including qPCR and 2-step qRT-PCR, in the SYBR Green I detection format. SYBR Green Premix provides high sensitivity, wide dynamic range and reproducibility for quantification.

The EZtime Real-Time PCR Premix (# FYT105-100P or # FYT106-100P) is a ready-to-use, 2× concentrated PCR premix that contains all reagents (except for primers, probe and template) needed for running real-time PCR (# FYT106-100P with ROX). Real-time PCR is performed by addition of various probes, such as Taq Man, Molecular Beacon, etc. This product combines the high performance Hotstart Taq with a buffer which provides good specificity, amplification efficiency for real-time PCR.

Features
    • Specificity :
      Hotstart Taq and the optimized buffer eliminate non-specific amplification and formation of primer dimers
    • Sensitivity :
      detects low copy number targets
    • Wide linear range :
      accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience :
    ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
Applications
  • Quantitative real-time PCR
  • Quantitative 2-step RT-PCR
  • Quick and accurate detection and quantification of target gene through real-time PCR
Quality Control
  • Error<0.01, 2.1>Efficiency>1.90, ∆CP<1
  • The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in qPCR for specificity, sensitivity and reproducibility using serial dilutions of control genomic DNA template.
Results

 

 

Specification
Store at 

 

# FYT103-100P (100 preps) 

Eztime Real-Time PCR Premix (2×, SYBR Green) 1.25 ml

# FYT103-400P (400 preps) 

Eztime Real-Time PCR Premix (2×, SYBR Green) 1.25ml×4

# FYT104 -100P (100 preps) 

Eztime Real-Time PCR Premix (2×, SYBR Green, ROX) 1.25 ml

# FYT104-400P (400 preps) 

Eztime Real-Time PCR Premix (2×, SYBR Green, ROX) 1.25 ml ×4

# FYT105-100P (100 preps) 

Eztime Real-Time PCR Premix (2×, TaqMan) 1.25ml

# FYT105-400P (400 preps) 

Eztime Real-Time PCR Premix (2×, TaqMan) 1.25 ml×4

# FYT106-100P (100 preps) 

Eztime Real-Time PCR Premix (2×, TaqMan, ROX) 1.25 ml

# FYT106-400P (400 preps 

Eztime Real-Time PCR Premix (2×, TaqMan, ROX) 1.25ml×4

Premix contains: 

■ Hotstart Taq DNA polymerase
■ SYBR Green real-time PCR Buffer
■ dNTP mix including dATP, dCTP, 
   dGTP, dTTP
■ 5 mM MgCl2