EZtime Fast Real-Time PCR Premix
Desciption
EZtime Fast Real-Time PCR Premix for SYBR® Green (#FYT108-100P/400P) is a ready-to-use, 2× concentrated premix reagent, containing all components except primers and template. It is formulated with a novel hot start Taq DNA polymerase, which is capable of catalyzing DNA amplification in a fast PCR mode. This special blend greatly shortens the running time of real-time quantitative PCR by around 1 hour when compared to traditional qPCR. In addition, it precisely meets current researchers’ needs for performing gene detection (qPCR) and quantification of gene expression (2-step qRT-PCR) in a high speed and/or high-throughput manner in addition to those basic requirements of high sensitivity, wide dynamic range, and good reproducibility. 

EZtime Fast Real-Time PCR Premix for TaqMan® Probe (#FYT110-100P/400P) is a ready-to-use, 2× concentrated premix reagent, containing all components except primers, probe, and template. It is formulated with a novel hot-start Taq DNA polymerase, which is capable of catalyzing DNA amplification in a fast PCR mode. This special blend greatly shortens the running time of real-time quantitative PCR by around 1 hour when compared to traditional qPCR. In addition, it precisely meets current researchers’ needs for performing gene detection (qPCR) and quantification of gene expression (2-step qRT-PCR) in a high speed and/or high-throughput manner in addition to those basic requirements of high sensitivity, wide dynamic range, and good reproducibility.

Features
• Fast : shortens the running time of real-time quantitative PCR by around 1 hour 
• Specificity : Hotstart Taq and the optimized buffer eliminates non-specific amplification and formation of primer dimers 
• Sensitivity : detects low copy number targets 
• Wide linear range : accurate quantification across 9 orders of magnitude 
• Reproducibility and convenience : ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
Applications
• Quantitative real-time PCR 
• Quantitative 2-step RT-PCR 
• Quick and accurate detection and quantification of target gene through real-time PCR
Quality Control
• Error<0.01, 2.1>Efficiency>1.90, ∆CP<1 
• The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in qPCR for specificity, sensitivity and reproducibility using serial dilutions of control genomic DNA template.
Specification
Store at 

 

# FYT108-100P (100 preps) 

EZtime Fast Real-Time PCR Premix 
(2×, SYBR Green, ROX)
1 ml

# FYT108-400P (400 preps) 

EZtime Fast Real-Time PCR Premix 
(2×, SYBR Green, ROX)
1 ml x 4

# FYT110-100P (100 preps) 

EZtime Fast Real-Time PCR Premix 
(2×, TaqMan, ROX)
1 ml

# FYT110-400P (400 preps) 

EZtime Fast Real-Time PCR Premix 
(2×, TaqMan, ROX)
1 ml x 4