HiFi DNA Polymerase
 
Description
HiFi DNA polymerase is ideal for amplification of DNA fragments that require
high-fidelity. High fidelity is achieved by an optimal blend of high performance
YEAtaq DNA polymerase and Accu DNA polymerase, a Pyrococcus proofreading
(3'→5' exonuclease activity ) enzyme. This formulation achieves greater yields
with higher fidelity than standard DNA polymerases such as Taq. HiFi is a
quality choice for sensitive applications. Because of the presence of Accu DNA
polymerase in the blend, dUTP, dITP and primers containing these nucleotides
should not be used in PCR because they hinder DNA synthesis.
HiFi DNA polymerase is offered in two different packages, one with dNTPs mix
and the other without. HiFi DNA Polymerase is also available in 2× ready-to-use
premix format with a green dye.
 
Features
• Robust amplification with minimal optimization.
• High yields of PCR products.
• Higher sensitivity compared to conventional Taq DNA Polymerase.
• Amplification of long targets up to 6 kb from genomic DNA.
• Generates 3’-dA overhangs.
• Incorporates modified nucleotides.
• The error rate of HiFi DNA Polymerase in PCR is 8.3×10-6 errors per nucleotide
per cycle.
 
Applications
• Cloning and expression
• Blunt end amplification products
• Mutation analysis
• Efficient & highly sensitive PCR use
• Routine PCR amplification of DNA fragments up to 6 kb from genomic DNA
• RT-PCR
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of
dNTPs into acid-insoluble material in 30 minutes at 72 °C.
 
Quality Control
• Nuclease activity is not detected after incubation of 1 μg λ/Hind III DNA with
5 units of HiFi DNA polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
• The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed
by appropriate tests. Functional test is performed by PCR.
Patterns/Disclaimer
Some applications in which this product can be used may be covered by
patents issued and applicable in certain countries. Because purchase of this
product does not include a license to perform any patented application, users
of this product may be required to obtain a patent license depending upon the
particular application and country in which the product is used.
Specification
Store at 

 

# FYT004-500U (500 units) 

Accu DNA Polymerase 100 μl (5 U/μl)
10× Reaction Buffer 2 ml
dNTPs Mix 200 μl (10 mM)

# FYT044-500U (500 units) 

Accu DNA Polymerase 100 μl (5 U/μl)
10× Reaction Buffer 2 ml

# FYT401-100P (100 preps) 

Accu DNA Polymerase Premix (2×) with Green dye 1.25 ml × 2

Storage Buffer 

50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers

10× Reaction Buffer* 

100 mM KCl, 20 mM MgSO4·7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA

* The reaction buffer is supplied as a 10× concentrate and should be diluted before use.