Accu DNA Polymerase
Description
Accu DNA Polymerase is purified from an E. coli strain carrying a plasmid with a cloned gene encoding a Pyrococcus Sp. DNA polymerase. The enzyme catalyzes the incorporation of nucleotides into dsDNA in the 5'→3' direction in the presence of Mg2+ at 70 ~ 80°C. Accu DNA Polymerase exhibits 3'→5' exonuclease (proofreading) activity, but has no detectable 5'→3' exonuclease activity. dUTP, dITP and primers containing these nucleotides should not be used in PCR with Accu DNA polymerase because they inhibit DNA synthesis

Accu DNA Polymerase in two different packages is offered by Yeastern Biotech, one with dNTPs mix and the other without. A PCR premix format is also available with or without a green dye.

Features
  • The error rate of Accu DNA polymerase in PCR is 2.6 × 10-6errors per nucleotide per cycle.
  • Eight times more accurate than Taq DNA polymerase.
  • Highly thermostable: remains 95% activity after 2 hours incubation at 95°C.
  • Generates blunt-end PCR products.
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
Applications
  • Cloning
  • Amplification
  • Mutation analysis
  • Accurate polymerization
  • Routine PCR amplification of DNA fragments up to 3 kb
  • RT-PCR
  • Blunt end amplification products
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Quality Control
  • Nuclease activity is not detected after incubation of 1 μg λ /Hind III DNA with 5 units of Accu DNA polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
  • The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate tests. Functional test is performed by PCR.
Patterns/Disclaimer
Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is use.

Results

 

Specification
Store at 

# FYT004-500U (500 units) 

Accu DNA Polymerase 100 μl (5 U/μl)
10× Reaction Buffer 2 ml
dNTPs Mix 200 μl (10 mM)

# FYT044-500U (500 units) 

Accu DNA Polymerase 100 μl (5 U/μl)
10× Reaction Buffer 2 ml

# FYT401-100P (100 preps) 

Accu DNA Polymerase Premix (2×) with Green dye 1.25 ml × 2

Storage Buffer 

50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers

10× Reaction Buffer* 

100 mM KCl, 20 mM MgSO4·7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA

* The reaction buffer is supplied as a 10× concentrate and should be diluted before use.