O’in 1 DNA Polymerase Premix
Description
O’in 1 DNA Polymerase Premix is an economical and ready-to-use premix, containing YEAtaq DNA Polymerase (# FYT001-500U), dNTP and all other reagents necessary for PCR, except DNA template and primers. It saves the time for preparing the master mix and reduces the risk of contamination from multiple pipetting steps. O’in 1 2× DNA Polymerase Premixes are also available with different non-interfering dyes for applications when loading dyes are desired. Users can choose from 5 different colors, including red, orange (mix of red and orange), violet, green (mix of blue and orange), and blue, depending on the preferred dye locations on the gel.

Optimal PCR conditions, including template and primer concentrations and PCR program, for gene of interest should be determined experimentally by the investigator from case to case .

Features
  • Convenient: taq DNA polymerase in a ready-to-use mix with or without dyes
  • Reproducible: lower contamination and pipetting error risk
Applications
  • Ready to use
  • Master mix
  • Suitable for economic screening
  • High throughput PCR
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Size of Dye (in 1% agarose gel)
  • Red: ~700 bp
  • Orange: ~50 bp
  • Blue: ~4000 bp
  • Violet: ~400 bp
Quality Control
  • Nuclease activity is not detected after incubation of 1 μg λ /Hind III DNA with 5 units of HiFi DNA Polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
  • The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate tests. Functional test is performed by PCR.
Patterns/Disclaimer
Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Results

Specification
Store at 

 

# FYT201-100P (100 preps) 

O’ in 1 DNA Polymerase Premix (1×) 1.25 ml x 4

# FYT201-96WL (96 wells) 

96-Well O’ in 1 DNA Polymerase Premix 23 μl x 96

# FYT202-100P (100 preps) 

O’ in 1 DNA Polymerase Premix (2×) 1.25 ml x 2

Store at 

 

# FYT203-100P 

O’ in 1 DNA Polymerase Premix (2×) with Red Dye 1.25 ml x 2

# FYT204-100P 

O’ in 1 DNA Polymerase Premix (2×) with Orange Dye 1.25 ml x 2

# FYT205-100P 

O’ in 1 DNA Polymerase Premix (2×) with Green Dye 1.25 ml x 2

# FYT206-100P 

O’ in 1 DNA Polymerase Premix (2×) with Blue Dye 1.25 ml x 2

# FYT207-100P 

O’ in 1 DNA Polymerase Premix (2×) with Violet Dye 1.25 ml x 2

1× O’in 1 DNA Polymerase Premix 

10 mM KCl , 2 mM MgSO4·7H2O, 20 mM Tris-HCl (pH 8.8), 0.1% Triton X-100, 10 mM (NH4)2SO4 , 0.1 mg/ml BSA, 0.2 mM dNTP mix, 50 U/ml YEAtaq DNA Polymerase, stabilizers.

2× O’in 1 DNA Polymerase Premix 

Two folds of all the above reagents