After reverse transcription, reactions are heated to 95°C for 10 minutes to inactivate the reverse transcriptase and simultaneously activate HotStart Taq DNA polymerase. This hot start to the PCR eliminates any nonspecific amplification products such as primer-dimers and reduces background smear, ensuring highly sensitive and reproducible RT-PCR.
- Fast and easy one-tube setup
- One-step RT-PCR of any RNA template without optimization
- Unique enzyme mix for high specificity and sensitivity
- Optimized reverse-transcription and amplification buffer
- Single-cell RT-PCR
- Gene-expression analysis