YB Rapid Ligation Kit
Description
Yeastern's yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5' – phosphate and the 3' – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. YB Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.
Features
  • Either sticky- or blunt-end, ligation can be achieved within 5 minutes
  • Reaction mixture can be used directly for bacterial transformation
Applications
  • Join double-strand DNA with cohesive or blunt termini
  • Join oligonucleotide linkers to DNA sequence with blunt end
  • Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids
Combo Products
Lyophilized yT4 DNA Ligase
An ambient-temperature-stable tube contains a glassified yT4 DNA ligase which yields a solution of yT4 DNA ligase when reconstituted to a final volume of 100 μl of deionized water.
Quality Control
Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities; Undetectable ribonuclease activity; Undetectable degradation of labeled oligonucleotide; Passed the test of the capability to join cohesive- and blunt-ended DNA fragments.
Specification
Store at 

# FYC003-100R (100 rxns) 

yT4 DNA Ligase 100 μl (3 U/μl)
10× Ligation Buffer A 200 μl
10× Ligation Buffer B 200 μl

Storage Buffer 

Tris-HCl (pH7.5) 20 mM
KCl 50 mM
DTT 1 mM
EDTA 0.1 mM
Glycerol stabilizers 50%

10× Ligation Buffer A 

Tris-HCl 0.4M
MgCl2 0.1 mM
DTT 0.1 mM
EDTA 5 mM
ATP (pH5.0 at 25°C) 5 mM
The performance of this buffer depends on the integrity of ATP. Store the buffer in small aliquots at -20°C to minimize degradation of ATP and DTT

10× Ligation Buffer B 

Buffer contains an enhancer which dramatically increases ligation efficiency for blunt end DNA