Yeastern's yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5' – phosphate and the 3' – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. YB Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.
- Either sticky- or blunt-end, ligation can be achieved within 5 minutes
- Reaction mixture can be used directly for bacterial transformation
- Join double-strand DNA with cohesive or blunt termini
- Join oligonucleotide linkers to DNA sequence with blunt end
- Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids
Lyophilized yT4 DNA Ligase
An ambient-temperature-stable tube contains a glassified yT4 DNA ligase which yields a solution of yT4 DNA ligase when reconstituted to a final volume of 100 μl of deionized water.
Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities; Undetectable ribonuclease activity; Undetectable degradation of labeled oligonucleotide; Passed the test of the capability to join cohesive- and blunt-ended DNA fragments.
# FYC003-100R (100 rxns)
|yT4 DNA Ligase||100 μl (3 U/μl)|
|10× Ligation Buffer A||200 μl|
|10× Ligation Buffer B||200 μl|
|Tris-HCl (pH7.5)||20 mM|
10× Ligation Buffer A
|ATP (pH5.0 at 25°C)||5 mM|
|The performance of this buffer depends on the integrity of ATP. Store the buffer in small aliquots at -20°C to minimize degradation of ATP and DTT|
10× Ligation Buffer B
Buffer contains an enhancer which dramatically increases ligation efficiency for blunt end DNA