ECOS™ 10B Competent Cells [DH10B]

Product overview

Description

DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.

While DH10B has been classically reported to be galgalK, the preliminary genome sequence for DH10B indicates that DH10B is actually galgalgalU+. Genome sequence indicates that DH10B is actually deoR+. The complete genome sequence has been published.

Features
  • Streptomycin resistant
  • Prevent DNA methylation
  • High transformation efficiency
Applications
  • Suitable for genomic DNA cloning
  • Allow large inserts
  • Cloning of methylated cytosine or adenine containing DNA
  • Blue/white screening

Technical / Specifications

Store at

Genotype
FendA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ
Specifications

# FYE507-10VL (10 preps)

Efficiency >5 x 107 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE507-80VL (80 preps)

Efficiency >5 x 107 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE508-10VL (10 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE508-80VL (80 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE507-96WL (96 preps)

Efficiency >1 x 107 cfu/μg
50 μl/well 96 wells

Support/ Documents

Manual ECOS Card