ECOS™ 10B Competent Cells [DH10B]
 
Strain: DH10B
Description
DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.

While DH10B has been classically reported to be galgalK, the preliminary genome sequence for DH10B indicates that DH10B is actually galgalgalU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence has been published.

Genotype
F-endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-
Features
  • Streptomycin resistant
  • Prevent DNA methylation
  • High transformation efficiency
Applications
  • Suitable for genomic DNA cloning
  • Allow large inserts
  • Cloning of methylated cytosine or adenine containing DNA
  • Blue/white screening
Efficiency
>5 x 107 cfu/ μg
Recommended for :

  • Subcloning
  • Genomic DNA cloning

>1 x 108 cfu/ μg
Recommended for :

  • Cloning of large insert plasmids
  • Genomic DNA cloning
 
Specification
Store at 

# FYE507-10VL (10 preps) 

Efficiency >5 x 107 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE507-80VL (80 preps) 

Efficiency >5 x 107 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE508-10VL (10 preps) 

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE508-80VL (80 preps) 

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE507-96WL (96 preps) 

Efficiency >1 x 107 cfu/μg
50 μl/well 96 wells