ECOS™ 21 Competent Cells [BL21(DE3)]
Strain: BL21 (DE3)
 
Description
This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Genotype
F- ompgal dcm lon hsdSB(rB- mB-) λ(DE3 [laclacUV5-T7 gene 1 indsamnin5])
Features
  • Lack of Lon and Omp proteases allows high-level protein expression and accumulation
  • Easy gene induction
Applications
  • IPTG induced gene expression in E. coli
  • Ideal for high level expression of non-toxic recombinant protein expression using T7-based expression vectors
  • Subcloning
Efficiency
>2 x 107 cfu/ μg
Recommended for :

  • recombinant protein expression
  • Subcloning
 
Specification
Store at 

# FYE207-5VL (5 preps) 

Efficiency >2 x 107 cfu/μg
100 μl/vial 5 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE207-40VL (40 preps) 

Efficiency >2 x 107 cfu/μg
100 μl/vial 40 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE207-96WL (96 preps) 

Efficiency >2 x 107 cfu/μg
50 μl/well 96wells