ECOS™ Blue Competent Cells [XL1-Blue]
Strain: XL1-Blue
 
Description
XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
Genotype
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F'proABlacIqZDM15 Tn10 (TetR)]
Features
  • A strain modified to be ideal for blue/white screening
  • Tetracycline resistant
Applications
  • Routine cloning application
  • Subcloning
  • cDNA library
  • Blue/white screening
Efficiency
>5 x 107 cfu/ μg
Recommended for:

  • Routine cloning and subcloning
  • Blue/white screening

>2 x 108 cfu/μg
Recommended for: 
Recommended for:

  • Routine cloning and subcloning
  • Blue/white screening

>5 x 108 cfu/ μg
Recommended for:

  • Routine cloning and subcloning
  • Blue/white screening
  • cDNA library
 
Specification
Store at 

# FYE107-10VL (10 preps) 

Efficiency >5 x 107 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE107-80VL (80 preps) 

Efficiency >5 x 107 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE108-10VL (10 preps) 

Efficiency >2 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE108-80VL (80 preps) 

Efficiency >2 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE109-10VL (10 preps) 

Efficiency >2 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE109-80VL (80 preps) 

Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE107-96WL (96 preps) 

Efficiency >5 x 107 cfu/μg
50 μl/well 96-wells

# FYE108-96WL (96 preps) 

Efficiency >2 x 108 cfu/μg
50 μl/well 96-wells

# FYE109-96WL (96 preps) 

Efficiency >5 x 108 cfu/μg
50 μl/well 96-wells