ECOS™ 9-5 Competent Cells [JM109]
Strain: JM109
 
Description
JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics. 

This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

Genotype
e14-(McrA-recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+supE44 relA1 D(lac-proAB) [F’ traD36 proAB lacIqZDM15]
Features
  • Rapid growing strain
  • Carries F' episome
Applications
  • Preparation of ssDNA
  • Construction of gene library
  • Subcloning
Efficiency
>1 x 108 cfu/ μg
Recommended for: 

  • High efficiency cloning
  • Cloning low abundance transcripts
  • General cloning
  • Gene library

>1 x 109 cfu/μg
Recommended for: 

  • Super high efficiency cloning
  • Cloning lowest abundance transcripts
  • Gene library

>5 x 107 cfu/ μg
Recommended for:

  • Subcloning
  • General cloning
Specification
Store at 

# FYE707-10VL (10 preps) 

Efficiency >5 x 107 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE707-80VL (80 preps) 

Efficiency >5 x 107 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE707-50-20VL (20 preps) 

Efficiency >5 x 107 cfu/μg
50 μl/vial 20 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE708-10VL (10 preps) 

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE708-80VL (80 preps) 

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE709-10VL (10 preps) 

Efficiency >5 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE709-80VL (80 preps) 

Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE707-96WL (96 preps) 

Efficiency >5 x 107 cfu/μg
50 μl/well 96-wells

# FYE708-96WL (96 preps) 

Efficiency >1 x 108 cfu/μg
50 μl/well 96-wells

# FYE709-96WL (96 preps) 

Efficiency >1 x 109 cfu/μg
50 μl/well 96-wells