ECOS™ 101 Competent Cells [DH5a]

 
Strain: DH5α

 

 
Description

 

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Genotype

 

endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+phosupE44 thi-1 Δ(lacZYA-argF)U169 Φ80 Δ(lacZ)M15 F-
Features

 

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.
Applications

 

  • Cloning and subcloning
  • Scale-up application
  • Blue/white screening
Efficiency

 

>1 x 108 cfu/ μg
Recommended for:

  • Subcloning
  • General cloning

>3 x 108 cfu/ μg 
Recommended for:

  • High efficiency cloning
  • Cloning low abundance transcripts
  • General cloning
  • Gene library

>1 x 109 cfu/ μg
Recommended for: 

  • Super high efficiency cloning
  • Cloning lowest abundance transcripts
  • Gene library
 
Specification
Store at 

 

# FYE678-10VL (10 preps) 

Efficiency >5 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE678-80VL (80 preps) 

Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE607-10VL (10 preps) 

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE607-80VL (80 preps) 

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE607-50-20VL (20 preps) 

Efficiency >1 x 108 cfu/μg
50 μl/vial 20 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE608-10VL (10 preps) 

Efficiency >1 x 109 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE608-80VL (80 preps) 

Efficiency >1 x 109 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE607-96WL (96 preps) 

Efficiency >1 x 108 cfu/μg
50 μl/well 96 wells

# FYE608-96WL (96 preps) 

Efficiency >3 x 108 cfu/μg
50 μl/well 96 wells

# FYE609-96WL (96 preps) 

Efficiency >1 x 109 cfu/μg
50 μl/well 96 wells