ECOS™ 101 Competent Cells [DH5a]

           

Product overview

Description

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

Features

Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.

Applications
  • Cloning and subcloning
  • Scale-up application
  • Blue/white screening

Technical / Specifications

Store at

Genotype
endA1 recA1 relA1 gyrA96 hsdR17(rk, mk+) phoA supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 Δ(lacZ)M15 F
Specifications

# FYE678-10VL (10 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE678-80VL (80 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE607-10VL (10 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE607-80VL (80 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE608-10VL (10 preps)

Efficiency >3 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE608-80VL (80 preps)

Efficiency >3 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE609-10VL (10 preps)

Efficiency >1 x 109 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE609-80VL (80 preps)

Efficiency >1 x 109 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE607-96WL (96 preps)

Efficiency >1 x 108 cfu/μg
50 μl/well 96 wells

# FYE608-96WL (96 preps)

Efficiency >3 x 108 cfu/μg
50 μl/well 96 wells

# FYE609-96WL (96 preps)

Efficiency >1 x 109 cfu/μg
50 μl/well 96 wells

Support/ Documents

Manual ECOS Card